MicroRNA expression in hepatocellular carcinoma after the eradication of chronic hepatitis virus C infection using interferon therapy
Aim Hepatocellular carcinoma (HCC) develops in up to 5% of patients after the successful treatment of chronic hepatitis C virus (HCV) infection using interferon therapy. The aim of this study was to characterize miRNA expression in liver tissues from patients who achieved a sustained viral response...
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Published in | Hepatology research Vol. 46; no. 3; pp. E26 - E35 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Blackwell Publishing Ltd
01.03.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Aim
Hepatocellular carcinoma (HCC) develops in up to 5% of patients after the successful treatment of chronic hepatitis C virus (HCV) infection using interferon therapy. The aim of this study was to characterize miRNA expression in liver tissues from patients who achieved a sustained viral response (SVR).
Methods
Seventy‐one patients with resected HCC were enrolled into the present study: 61 HCC from patients with continuously infected HCV (HCV‐HCC) and 10 from patients who had achieved SVR (SVR‐HCC). We also included non‐tumor tissues (SVR‐NT) from four patients with SVR‐HCC, and liver tissue (SVR‐CH) from four SVR patients without HCC. Total RNA was extracted from liver samples. The miRNA expression patterns were analyzed using microarrays. In addition, target gene expression was quantified after miRNA overexpression in HEK293 cells.
Results
We could discriminate between SVR‐HCC and HCV‐HCC with 75.36% accuracy using the expression pattern of six specific miRNA. The expression levels of 37 miRNA were significantly lower in HCV‐HCC than in SVR‐HCC, whereas the expression of 25 miRNA was significantly higher in HCV‐HCC than SVR‐HCC (P < 1.0E‐05). The expression of thrombospondin 1 was regulated in an opposing manner by miR‐30a‐3p in SVR‐HCC and HCV‐HCC. In non‐tumor tissues, the expression pattern of seven miRNA could distinguish between SVR‐CH and SVR‐NT with 87.50% accuracy.
Conclusion
Comprehensive miRNA expression analyses could not only differentiate between SVR‐HCC and HCV‐HCC but also forecast hepatocarcinogenesis after achieving SVR. |
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Bibliography: | ark:/67375/WNG-0LNWDCT9-9 istex:E5E663C123899B60BBCD70F578B4C6BBE8046CD0 ArticleID:HEPR12518 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1386-6346 1872-034X |
DOI: | 10.1111/hepr.12518 |