Structural basis of α‐amylase activation by chloride

• Published in: Protein science Vol. 11; no. 6; pp. 1435 - 1441
• Main Authors: Aghajari, Nushin, Feller, Georges, Gerday, Charles, Haser, Richard
• Format: Journal Article Web Resource
• Language: English
• Published: Bristol Cold Spring Harbor Laboratory Press 01.06.2002; Wiley; Cold Spring Harbor Lab Press
• Subjects: Allosteric activation; alpha-amylase; alpha-Amylases - chemistry; alpha-Amylases - genetics; alpha-Amylases - metabolism; Amino Acid Substitution; Binding Sites - genetics; Biochemistry, biophysics & molecular biology; Biochemistry, Molecular Biology; Biochimie, biophysique & biologie moléculaire; catalysis; chloride ion; Chlorides - pharmacology; crystal structures; Crystallization; Crystallography, X-Ray; Enzyme Activation - drug effects; family 13 glycosyl hydrolases; Life Sciences; monovalent anions; Nitrates - pharmacology; Pseudoalteromonas - enzymology; psychrophilic; Sciences du vivant; Static Electricity; α‐amylase
• ISSN: 0961-8368; 1469-896X; 1469-896X
• DOI: 10.1110/ps.0202602

To further investigate the mechanism and function of allosteric activation by chloride in some α‐amylases, the structure of the bacterial α‐amylase from the psychrophilic micro‐organism Pseudoalteromonas haloplanktis in complex with nitrate has been solved at 2.1 A˚, as well as the structure of the mutants Lys300Gln (2.5 A˚) and Lys300Arg (2.25 A˚). Nitrate binds strongly to α‐amylase but is a weak activator. Mutation of the critical chloride ligand Lys300 into Gln results in a chloride‐independent enzyme, whereas the mutation into Arg mimics the binding site as is found in animal α‐amylases with, however, a lower affinity for chloride. These structures reveal that the triangular conformation of the chloride ligands and the nearly equatorial coordination allow the perfect accommodation of planar trigonal monovalent anions such as NO3−, explaining their unusual strong binding. It is also shown that a localized negative charge such as that of Cl−, rather than a delocalized charge as in the case of nitrate, is essential for maximal activation. The chloride‐free mutant Lys300Gln indicates that chloride is not mandatory for the catalytic mechanism but strongly increases the reactivity at the active site. Disappearance of the putative catalytic water molecule in this weakly active mutant supports the view that chloride helps to polarize the hydrolytic water molecule and enhances the rate of the second step in the catalytic reaction.