BOLD and CBV-weighted functional magnetic resonance imaging of the rat somatosensory system

• Published in: Magnetic resonance in medicine Vol. 55; no. 2; pp. 316 - 324
• Main Authors: Keilholz, Shella D., Silva, Afonso C., Raman, Mira, Merkle, Hellmut, Koretsky, Alan P.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.02.2006
• Subjects: 11.7 Tesla MRI; Animals; BOLD; Brain Mapping - methods; cerebral blood volume; Cerebrovascular Circulation - physiology; Contrast Media; Dextrans; Electric Stimulation; Ferrosoferric Oxide; fMRI; Iron; Magnetic Resonance Imaging - methods; Magnetite Nanoparticles; Male; Oxides; Oxygen - blood; Rats; Rats, Sprague-Dawley; Somatosensory Cortex - physiology; somatosensory stimulation
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.20744

A multislice spin echo EPI sequence was used to obtain functional MR images of the entire rat brain with blood oxygenation level dependent (BOLD) and cerebral blood volume (CBV) contrast at 11.7 T. Maps of activation incidence were created by warping each image to the Paxinos rat brain atlas and marking the extent of the activated area. Incidence maps for BOLD and CBV were similar, but activation in draining veins was more prominent in the BOLD images than in the CBV images. Cerebellar activation was observed along the surface in BOLD images, but in deeper regions in the CBV images. Both effects may be explained by increased signal dropout and distortion in the EPI images after administration of the ferumoxtran‐10 contrast agent for CBV fMRI. CBV‐weighted incidence maps were also created for 10, 20, and 30 mg Fe/kg doses of ferumoxtran‐10. The magnitude of the average percentage change during stimulation increased from 4.9% with the 10 mg Fe/kg dose to 8.7% with the 30‐mg Fe/kg dose. Incidence of activation followed a similar trend. Magn Reson Med, 2006. Published 2005 Wiley‐Liss, Inc.