Characterization of the programmed cell death induced by metabolic products of Alternaria alternata in tobacco BY-2 cells

• Published in: Physiologia plantarum Vol. 141; no. 2; pp. 117 - 129
• Main Authors: Cheng, Dan-Dan, Jia, Yu-Jiao, Gao, Hui-Yuan, Zhang, Li-Tao, Zhang, Zi-Shan, Xue, Zhong-Cai, Meng, Qing-Wei
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2011; Blackwell
• Subjects: Adenosine; Adenosine - metabolism; Adenosine Triphosphate - metabolism; Alternaria - chemistry; Alternaria alternata; Apoptosis; ATP; Atrophy; Biological and medical sciences; Catalase; Catalase - metabolism; Cell death; Cell Death - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Cells, Cultured; Chloroplasts; Chromatin; Condensation; Cytoplasm; Data processing; DNA fragmentation; Electron transport; Electron transport chain; Fundamental and applied biological sciences. Psychology; Hydrogen Peroxide - metabolism; Mitochondria; Nicotiana - cytology; Nicotiana - drug effects; Phytopathology. Animal pests. Plant and forest protection; Plant cells; Plant Extracts - pharmacology; Plant physiology and development; Reactive oxygen species; Reactive Oxygen Species - metabolism; Tobacco; Fungi; Characterization; Plant pathogen; Plant physiology; Cell death; Fungi Imperfecti; Alternaria alternata; Metabolism; Cell; Apoptosis
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/j.1399-3054.2010.01422.x

Alternaria alternata has received considerable attention in current literature and most of the studies are focused on its pathogenic effects on plant chloroplasts, but little is known about the characteristics of programmed cell death (PCD) induced by metabolic products (MP) of A. alternata, the effects of the MP on mitochondrial respiration and its relation to PCD. The purpose of this study was to explore the mechanism of MP-induced PCD in non-green tobacco BY-2 cells and to explore the role of mitochondrial inhibitory processes in the PCD of tobacco BY-2 cells. MP treatment led to significant cell death that was proven to be PCD by the concurrent cytoplasm shrinkage, chromatin condensation and DNA laddering observed in the cells. Moreover, MP treatment resulted in the overproduction of reactive oxygen species (ROS), rapid ATP depletion and a respiratory decline in the tobacco BY-2 cells. It was concluded that the direct inhibition of the mitochondrial electron transport chain (ETC), alternative pathway (AOX) capacity and catalase (CAT) activity by the MP might be the main contributors to the MP-induced ROS burst observed in tobacco BY-2 cells. The addition of adenosine together with the MP significantly inhibited ATP depletion without preventing PCD; however, when the cells were treated with the MP plus CAT, ROS overproduction was blocked and PCD did not occur. The data presented here demonstrate that the ROS burst played an important role in MP-induced PCD in the tobacco BY-2 cells.