Association of small CAB-like proteins (SCPs) of Synechocystis sp. PCC 6803 with Photosystem II

• Published in: Photosynthesis research Vol. 95; no. 2-3; pp. 135 - 145
• Main Authors: Kufryk, Galyna, Hernandez-Prieto, Miguel A, Kieselbach, Thomas, Miranda, Helder, Vermaas, Wim, Funk, Christiane
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.02.2008; Springer Netherlands; Springer Nature B.V
• Subjects: Amino Acid Sequence; Antenna; Bacteria; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Base Sequence; Biochemistry; Biomedical and Life Sciences; Blotting, Northern; Blotting, Western; Chlorophyll-binding protein; Cyanobacteria; DNA Primers; Early-light induced proteins (ELIPs); Electrophoresis, Polyacrylamide Gel; Genes, Plant; High-light induced proteins (HLIPs); Life Sciences; Light-harvesting complex; Molecular Sequence Data; Photosynthesis; Photosystem II Protein Complex - metabolism; Plant biology; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Protein Binding; Proteins; Regular Paper; RNA, Plant - genetics; Sequence Homology, Amino Acid; Synechocystis; Synechocystis - genetics; Synechocystis - metabolism; Synechocystis sp. PCC 6803; Tandem Mass Spectrometry; Chlorophyll-binding protein; High-light induced proteins (HLIPs); Cyanobacteria; Early-light induced proteins (ELIPs); sp. PCC 6803; Light-harvesting complex; Antenna
• ISSN: 0166-8595; 1573-5079; 1573-5079
• DOI: 10.1007/s11120-007-9244-3

The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress conditions but their exact function remains largely unknown. Recently we showed association of ScpD with light-stressed, monomeric Photosystem II in Synechocystis sp. PCC 6803 (Yao et al. J Biol Chem 282:267-276, 2007). Here we show that ScpB associates with Photosystem II at normal growth conditions. Moreover, upon introduction of a construct into Synechocystis so that ScpB is expressed continuously under normal growth conditions, ScpE was detected under non-stressed conditions as well, and was copurified with tagged ScpB and Photosystem II. We also report on a one-helix protein, Slr1544, that is somewhat similar to the SCPs and whose gene is cotranscribed with that of ScpD; Slr1544 is another member of the extended light-harvesting-like (Lil) protein family, and we propose to name it LilA.