Involvement of Regulatory Interactions among Global Regulators GlxR, SugR, and RamA in Expression of ramA in Corynebacterium glutamicum

• Published in: Journal of Bacteriology Vol. 195; no. 8; pp. 1718 - 1726
• Main Authors: Toyoda, Koichi, Teramoto, Haruhiko, Gunji, Wataru, Inui, Masayuki, Yukawa, Hideaki
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.04.2013
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Binding Sites; carbon; Cells; Corynebacterium glutamicum; Corynebacterium glutamicum - genetics; Corynebacterium glutamicum - metabolism; DNA, Bacterial; DNA, Intergenic; Down-Regulation; Escherichia coli - genetics; Escherichia coli - metabolism; Gene expression; Gene Expression Regulation, Bacterial - physiology; genes; genetic background; Gram-positive bacteria; metabolism; mutants; Mutation; Plasmids; promoter regions; Promoter Regions, Genetic; Protein Binding; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; transcription (genetics); transcription factors; Transcription, Genetic
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/JB.00016-13

The central carbon metabolism genes in Corynebacterium glutamicum are under the control of a transcriptional regulatory network composed of several global regulators. It is known that the promoter region of ramA, encoding one of these regulators, interacts with its gene product, RamA, as well as with the two other regulators, GlxR and SugR, in vitro and/or in vivo. Although RamA has been confirmed to repress its own expression, the roles of GlxR and SugR in ramA expression have remained unclear. In this study, we examined the effects of GlxR binding site inactivation on expression of the ramA promoter-lacZ fusion in the genetic background of single and double deletion mutants of sugR and ramA. In the wild-type background, the ramA promoter activity was reduced to undetectable levels by the introduction of mutations into the GlxR binding site but increased by sugR deletion, indicating that GlxR and SugR function as the transcriptional activator and repressor, respectively. The marked repression of ramA promoter activity by the GlxR binding site mutations was largely compensated for by deletions of sugR and/or ramA. Furthermore, ramA promoter activity in the ramA-sugR double mutant was comparable to that in the ramA mutant but was significantly higher than that in the sugR mutant. Taken together, it is likely that the level of ramA expression is dynamically balanced by GlxR-dependent activation and repression by RamA along with SugR in response to perturbation of extracellular and/or intracellular conditions. These findings add multiple regulatory loops to the transcriptional regulatory network model in C. glutamicum.