The usefulness of immunohistochemistry for phosphohistone H3 as a prognostic factor in myxoid liposarcoma

• Published in: Scientific reports Vol. 13; no. 1; p. 4733
• Main Authors: Takazawa, Akira, Yoshimura, Yasuo, Okamoto, Masanori, Tanaka, Atsushi, Kito, Munehisa, Aoki, Kaoru, Uehara, Takeshi, Takahashi, Jun, Kato, Hiroyuki, Nakayama, Jun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.03.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/67; 692/4028; Adult; Biomarkers, Tumor - metabolism; Histones - metabolism; Humanities and Social Sciences; Humans; Immunohistochemistry; Ki-67 Antigen - analysis; Liposarcoma; Liposarcoma, Myxoid; Medical prognosis; multidisciplinary; Multivariate analysis; Phosphorylation; Prognosis; Regression analysis; Science; Science (multidisciplinary); Survival; Tumor cells
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-31896-y

Myxoid liposarcoma (MLS) is a common subtype of liposarcoma. Although the prognosis is generally good, there are factors known to be associated with poor prognosis. Accurate indices are important to predict prognosis. We aimed to assess the usefulness of immunohistochemistry for phosphohistone H3 (PHH3) as a potential biomarker in comparison with Ki-67 antigen and other prognostic factors. Twenty-five patients with MLS were evaluated. Age, sex, depth of tumor, tumor size, surgical margin, oncological outcome, histological grade, presence of necrosis, proportion of round cell component (RC%), PHH3 index, and Ki-67 index were examined. Prognostic factors of the examination criteria were statistically analyzed, survival rate analyses were performed using the Kaplan–Meier method, and multivariate analysis was performed using Cox proportional-hazard regression analysis. The number of PHH3-positive tumor cells and the PHH3 and Ki-67 indices demonstrated a statistical correlation with the prognosis of MLS in univariate analysis ( P  < 0.001, P  < 0.001, P  = 0.01, respectively). PHH3 index and RC% were significant factors in multivariate analysis ( P  = 0.03, P  = 0.02). The immunohistochemistry of PHH3 may be associated with prognosis and could serve as a valid criterion of histological grade in MLS.