Inhibitory effect of three C-glycosylflavonoids from Cymbopogon citratus (Lemongrass) on human low density lipoprotein oxidation

This study assessed the inhibitory effect of three C-glycosylflavonoids from Cymbopogon citratus leaves--isoorientin (1), swertiajaponin (2) and isoorientin 2"-Orhamnoside (3)--on human LDL oxidation. Isolated LDL was incubated with compounds 1-3 and the kinetics of lipid peroxidation were asse...

Full description

Saved in:
Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 14; no. 10; pp. 3906 - 3913
Main Authors Orrego, Roxana, Leiva, Elba, Cheel, José
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 30.09.2009
Molecular Diversity Preservation International
Subjects
Anticholesteremic Agents - chemistry
Anticholesteremic Agents - pharmacology
antioxidant
Antioxidants
Atherosclerosis
Cardiovascular disease
Cholesterol, LDL - drug effects
Cholesterol, LDL - metabolism
Copper
Cymbopogon - chemistry
Cymbopogon citratus
Flavonoids
Flavonoids - chemistry
Flavonoids - pharmacology
Humans
lemongrass
Lipid peroxidation
Lipid Peroxidation - drug effects
Lipids
Low density lipoprotein
Luteolin - chemistry
Luteolin - pharmacology
Oxidation
oxidized LDL
Online AccessGet full text

Cover

More Information
Summary:This study assessed the inhibitory effect of three C-glycosylflavonoids from Cymbopogon citratus leaves--isoorientin (1), swertiajaponin (2) and isoorientin 2"-Orhamnoside (3)--on human LDL oxidation. Isolated LDL was incubated with compounds 1-3 and the kinetics of lipid peroxidation were assessed by conjugated diene and malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS) formation after addition of copper ions. Significant differences (p < 0.05) between the lag time phase of the control and the lag time phase in the presence of the compounds 1 (0.25 microM) and 2 (0.50 microM) were observed. After five hours of incubation all three compounds showed a significant inhibitory effect on MDA-TBARS formation with respect to the control. After six hours of incubation only compound 1 kept a remarkable antioxidant effect. This study demonstrates that isoorientin (1) is an effective inhibitor of in vitro LDL oxidation. As oxidative damage to LDL is a key event in the formation of atherosclerotic lesions, the use of this natural antioxidant may be beneficial to prevent or attenuate atherosclerosis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules14103906