Determining optimum conditions for lipase-catalyzed synthesis of triethanolamine (TEA)-based esterquat cationic surfactant by a Taguchi robust design method

• Published in: Molecules (Basel, Switzerland) Vol. 16; no. 6; pp. 4672 - 4680
• Main Authors: Masoumi, Hamid Reza Fard, Kassim, Anuar, Basri, Mahiran, Abdullah, Dzulkifly Kuang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.06.2011; MDPI
• Subjects: Arrays; Biosynthesis; Catalysis; cationic surfactant; Cations - chemistry; Chemistry - methods; Conversion; Design of experiments; Design techniques; Enzymes; esterquat; Ethanolamines - chemistry; Lipase - metabolism; Models, Statistical; Oleic Acid - chemistry; optimization; Optimization techniques; Reproducibility of Results; Research methodology; Software; Surface-Active Agents - chemical synthesis; Surfactants; Taguchi design; Taguchi methods; Variance analysis
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules16064672

A Taguchi robust design method with an L₉ orthogonal array was implemented to optimize experimental conditions for the biosynthesis of triethanolamine (TEA)-based esterquat cationic surfactants using an enzymatic reaction method. The esterification reaction conversion% was considered as the response. Enzyme amount, reaction time, reaction temperature and molar ratio of substrates, [oleic acid: triethanolamine (OA:TEA)] were chosen as main parameters. As a result of the Taguchi analysis in this study, the molar ratio of substrates was found to be the most influential parameter on the esterification reaction conversion%. The amount of enzyme in the reaction had also a significant effect on reaction conversion%.