An electrochemical aptasensor for methylamphetamine rapid detection by single-on mode based on competition with complementary DNA

• Published in: Scientific reports Vol. 14; no. 1; p. 9279
• Main Authors: Chang, Wenzhuo, Zheng, Zhixiang, Ma, Yongjun, Du, Yongling, Shi, Xuezhao, Wang, Chunming
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.04.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 639/638/11; 639/638/11/872; 639/638/161/886; Amphetamines; Aptamer; Aptamers; Aptamers, Nucleotide - chemistry; Biosensing Techniques - methods; Chemical engineering; Chromatography; Competition; Competitive; Complementary DNA; DNA, Complementary - genetics; Drug use; Electrochemical Techniques - methods; Electrochemistry; Electrodes; Electrons; Gold; Gold - chemistry; Humanities and Social Sciences; Humans; Limit of Detection; Mass spectrometry; Mental disorders; Methamphetamine; Methamphetamine - analysis; Methamphetamine - urine; Methods; Methylamphetamine (MAMP); Methylene blue; Methylene Blue - chemistry; multidisciplinary; Point of care testing; Point-of-care testing (POCT); Saliva; Saliva - chemistry; Saliva - metabolism; Science; Science (multidisciplinary); Scientific imaging; Sensors; Urine; Voltammetry; Point-of-care testing (POCT); Competitive; Aptamer; Complementary DNA; Methylamphetamine (MAMP)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-59505-6

A simple and rapid electrochemical sensing method with high sensitivity and specificity of aptamers was developed for the detection of methylamphetamine (MAMP). A short anti-MAMP thiolated aptamer (Apt) with a methylene blue (MB) probe at 3ʹ-end was immobilized on the surface of a gold electrode (MB-Apt-S/GE). The electrochemical signal appeared when MAMP presenting in the sample solution competed with cDNA for binding with MB-Apt-S. Under optimized conditions, the liner range of this signal-on electrochemical aptasensor for the detection of MAMP achieved from 1.0 to 10.0 nmol/L and 10.0–400 nmol/L. LOD 0.88 nmol/L were obtained. Satisfactory spiked recoveries of saliva and urine were also obtained. In this method, only 5 min were needed to incubate before the square wave voltammetry (SWV) analysis, which was much more rapid than other electrochemical sensors, leading to a bright and broad prospect for the detection of MAMP in biological sample. This method can be used for on-site rapid detection on special occasions, such as drug driving scenes, entertainment venues suspected of drug use, etc.