A comparative identification of ochratoxin A in longan fruit pulp by high performance liquid chromatography-fluorescence detection and electron spray ionization-mass spectrometry

Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-pha...

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Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 15; no. 2; pp. 680 - 688
Main Authors Li, Jing, Xie, Haihui, Yang, Bao, Dong, Xinhong, Feng, Linyan, Chen, Feng, Jiang, Yueming
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 29.01.2010
Molecular Diversity Preservation International
Subjects
Chromatography
Chromatography, High Pressure Liquid - methods
ESI-MS
Fluorescence
fruit
Fruit - chemistry
Fruits
Fungal infections
HPLC-FD
longan
Mass spectrometry
Methods
ochratoxin A
Ochratoxins - analysis
Ochratoxins - chemistry
Pathogens
Plant Extracts - chemistry
pulp
Reference Standards
Retention
Sapindaceae - chemistry
Scientific imaging
solid-phase extraction
Spectrometry, Mass, Electrospray Ionization - methods
China
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Summary:Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules15020680