Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae

• Published in: Experimental gerontology Vol. 48; no. 10; pp. 1006 - 1013
• Main Authors: Delaney, Joe R., Murakami, Christopher, Chou, Annie, Carr, Daniel, Schleit, Jennifer, Sutphin, George L., An, Elroy H., Castanza, Anthony S., Fletcher, Marissa, Goswami, Sarani, Higgins, Sean, Holmberg, Mollie, Hui, Jessica, Jelic, Monika, Jeong, Ki-Soo, Kim, Jin R., Klum, Shannon, Liao, Eric, Lin, Michael S., Lo, Winston, Miller, Hillary, Moller, Richard, Peng, Zhao J., Pollard, Tom, Pradeep, Prarthana, Pruett, Dillon, Rai, Dilreet, Ros, Vanessa, Schuster, Alex, Singh, Minnie, Spector, Benjamin L., Wende, Helen Vander, Wang, Adrienne M., Wasko, Brian M., Olsen, Brady, Kaeberlein, Matt
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.10.2013
• Subjects: Animals; Caloric Restriction; Calorie restriction; Cell Division - physiology; Chronological lifespan; Culture Techniques - methods; Dietary restriction; Flow Cytometry; Glucose; Glucose - metabolism; Membrane Potential, Mitochondrial - physiology; Mitochondria; Mitochondria - physiology; Replicative lifespan; Reproduction - physiology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - physiology; Time Factors; Dietary restriction; Mitochondria; Chronological lifespan; Calorie restriction; Replicative lifespan; Glucose; Caloric restriction
• ISSN: 0531-5565; 1873-6815
• DOI: 10.1016/j.exger.2012.12.001

Chronological aging of budding yeast cells results in a reduction in subsequent replicative life span through unknown mechanisms. Here we show that dietary restriction during chronological aging delays the reduction in subsequent replicative life span up to at least 23days of chronological age. We further show that among the viable portion of the control population aged 26days, individual cells with the lowest mitochondrial membrane potential have the longest subsequent replicative lifespan. These observations demonstrate that dietary restriction modulates a common molecular mechanism linking chronological and replicative aging in yeast and indicate a critical role for mitochondrial function in this process. ► DR delays the reduction in replicative lifespan associated with chronological age. ► MMP during chronological aging predicts subsequent replicative lifespan. ► Chronologically aged cells with highest MMP cannot complete a cell division. ► Chronologically aged cells with lowest MMP maintain a full replicative lifespan.