The isolation of exopolysaccharide-producing lactic acid bacteria from lontar (Borassus flabellifer L.) sap

• Published in: Iranian journal of microbiology Vol. 12; no. 5; pp. 437 - 444
• Main Authors: Ma'unatin, Anik, Harijono, Harijono, Zubaidah, Elok, Rifa'i, Muhaimin
• Format: Journal Article
• Language: English
• Published: Iran Tehran University of Medical Sciences 01.10.2020
• Subjects: Borassus flabellifer; Exopolysaccharide; Fructobacillus fructosus; Lactic acid bacteria; Leuconostoc mesenteroides; Original; Borassus flabellifer; Leuconostoc mesenteroides; Lactic acid bacteria; Fructobacillus fructosus; Exopolysaccharide
• ISSN: 2008-3289; 2008-4447
• DOI: 10.18502/ijm.v12i5.4605

Lontar ( L.) is widely grown in Indonesia and one of its products is palm sap. Palm sap contains a high level of sugar, making it suitable as a medium to increase the lactic acid bacteria (LAB) production of exopolysaccharides (EPS). This study aimed to isolate the EPS-producing LAB from palm sap and evaluate its EPS production. LAB isolation was carried out on MRS agar containing 0.5% CaCO . The screening and production of EPS were carried out on MRS media supplemented with 10% sucrose. The molecular identification of the selected EPS-producing LAB was based on 16S rDNA. A quantitative analysis of EPS polymer dry mass and total sugar was conducted using one-way ANOVA. In this study, five EPS-producing LABs were found: N4, N5, N7, N9, and N10. The highest EPS yield in liquid media was 10.997 ± 1.591 g/L by N7, whereas the lowest was 4.505 ± 0.459 g/L by N10. This study found strains as EPS producers that have never been reported before.