Low-vacuum SEM imaging and viability test of L929 cells exposed to a Euro 6 diesel exhaust gas mixture in a BAT-CELL chamber in comparison with hydrocarbons emission

• Published in: Scientific reports Vol. 14; no. 1; pp. 12883 - 11
• Main Authors: Kęska, Aleksandra, Rusak, Agnieszka, Włostowski, Radosław, Dziemieszkiewicz, Mikołaj, Szymlet, Natalia
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.06.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 639/166/988; 704/158; Air Pollutants - analysis; Air Pollutants - toxicity; Animals; Apoptosis; Cell death; Cell Line; Cell morphology; Cell proliferation; Cell Survival - drug effects; Diesel engines; Driving ability; Electron microscopes; Emission; Emissions; ESEM; Fumes; Gas chromatography; Gases; Humanities and Social Sciences; Hydrocarbons; Hydrocarbons - toxicity; Inflammation; Internal combustion engine; Mice; Microscopy, Electron, Scanning; multidisciplinary; Scanning electron microscopy; Science; Science (multidisciplinary); Toxicity; Vehicle emissions; Vehicle Emissions - analysis; Vehicle Emissions - toxicity; Emission; Gas chromatography; Internal combustion engine; ESEM; Cell morphology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-63560-4

Exhaust emissions, which count among the most common causes of premature death worldwide, can cause irreversible changes in cells, leading to their damage or degeneration. In this research, L929 line cells were observed after exposure in the BAT-CELL chamber to exhaust gases emitted from a Euro 6 compression-ignition engine. Real road traffic conditions were simulated, taking into account air resistance while driving at speeds of 50 km/h, 120 km/h and idling engine. Morphological analysis of the cells was performed using an environmental scanning electron microscope. It has been observed that diesel exhaust fumes can cause inflammation, which can induce apoptosis or leads to necrotic cell death. The impact of the vehicle exhaust gases can inhibit cell proliferation by almost three times. Moreover, a correlation has been observed between the speed of the inflammatory reaction in cells and the presence of specific hydrocarbon compounds that determine the toxicity of exhaust gases. Research has shown that the toxicity of the emitted exhaust gases has been the highest at the driving speed of 120 km/h. In order to reduce the harmful effects of exhaust emissions, ecological alternatives and the supplementation of legal provisions regarding the compounds subject to limitation are necessary.