Chromosome-level genome assembly of Megachile lagopoda (Linnaeus, 1761) (Hymenoptera: Megachilidae)

• Published in: Scientific data Vol. 11; no. 1; pp. 1171 - 7
• Main Authors: Zhang, Dan, Jin, Jianfeng, Niu, Zeqing, Orr, Michael C., Zhang, Feng, Ferrari, Rafael R., Wu, Qingtao, Zhou, Qingsong, Da, Wa, Luo, Arong, Zhu, Chaodong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.10.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/181/2480; 631/181/735; Animals; Bees - genetics; China; Chromosomes; Chromosomes, Insect - genetics; Data Descriptor; Evolution; Evolutionary genetics; Genes; Genome, Insect; Genomes; Genomics; Humanities and Social Sciences; Megachile lagopoda; Megachilidae; multidisciplinary; Plant reproduction; Science; Science (multidisciplinary); Zoology; China
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/s41597-024-04028-y

Megachile is one of the largest bee genera, including nearly 1,500 species, but very few chromosome-level assemblies exist for this group or the family Megachilidae. Here, we report the chromosome-level genome assembly of Megachile lagopoda collected from Xizang, China. Using PacBio CLR long reads and Hi-C data, we assembled a genome of 256.83 Mb with 96.08% of the assembly located on 16 chromosomes. Our assembly contains 266 scaffolds, with a scaffold N50 length of 15.6 Mb, and BUSCO completeness of 99.20%. We masked 27.10% (69.61 Mb) of the assembly as repetitive elements, identified 459 non-coding RNAs, and predicted 11,157 protein-coding genes. This high-quality genome of M. lagopoda represents an important step forward for our knowledge of megachilid genomics and bee evolution overall.