Extraction, characterization of Angelica sinensis polysaccharides and modulatory effect of the polysaccharides and Tai Chi exercise on oxidative injury in middle-aged women subjects

• Published in: Carbohydrate polymers Vol. 77; no. 2; pp. 384 - 388
• Main Authors: Juan, Jiang, YingJie, Guo, AiJun, Niu
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 10.06.2009; Elsevier
• Subjects: adults; Angelica; Angelica sinensis; Angelica sinensis polysaccharides; Antioxidant; antioxidant activity; Applied sciences; bioactive properties; Biological and medical sciences; chemical composition; China; Exact sciences and technology; exercise; General and cellular metabolism. Vitamins; herbal medicines; HPLC; Immunity; Immunomodulators; Medical sciences; Middle-aged women; Miscellaneous; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; polysaccharides; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Starch and polysaccharides; Tai Chi; traditional medicine; women; China; Tai Chi; Middle-aged women; Angelica sinensis polysaccharides; Antioxidant; Immunity; HPLC; Oxidative stress; Molecular structure; Superoxide dismutase; Medicinal plant; Interleukin 6; Chinese medicine; Enzymatic activity; Catalase; Folk medicine; Dicotyledones; Angiospermae; Angelica; Woman; Tumor necrosis factor α; Gymnastics; Human; Glutathione peroxidase; Middle age; Enzyme; Interleukin 1β; Experimental study; Biological activity; Immunomodulator; Peroxidases; Martial art; Plant origin; Oside polymer; Spermatophyta; Oxidoreductases; Umbelliferae; Comparative study
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2009.01.010

The present study was designed to investigate the effect of Angelica sinensis polysaccharides (ASP) and Tai Chi exercise on free radical generation and lipid peroxidation and its modulatory effect on immunity activity in middle-aged women subjects at a dosage levels of 125 mg/kg. ASP was characterized by high performance liquid chromatography (HPLC). HPLC analysis showed that ASP was composed of eight kinds of monosaccharides, namely mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose in molar ratios of 1.2:4.5:1:10.5:17.8:37.5:8.7:4.9. Antioxidant activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) and lipid peroxidation levels in both ASP and Tai Chi exercise groups were significantly enhanced or reduced compared to the control group. In addition, immunity activities such as serum vascular cell adhesion molecule-1 (s-VCAM-1), interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) levels in both ASP and Ta Chi exercise groups were significantly reduced compared to the control group. It can be concluded that ASP intake and Tai Chi exercise decrease oxidant stress induced injury and improve immunity activities in middle-aged women subjects, indicating a obvious healthy promotion effect.