Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia
Zika is a vector-borne disease caused by an arbovirus (ZIKV) and overwhelmingly transmitted by Ae. aegypti . This disease is linked to adverse fetal outcomes, mostly microcephaly in newborns, and other clinical aspects such as acute febrile illness and neurologic complications, for example, Guillain...
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Published in | Frontiers in cellular and infection microbiology Vol. 12; p. 900608 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
07.07.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Zika is a vector-borne disease caused by an arbovirus (ZIKV) and overwhelmingly transmitted by
Ae. aegypti
. This disease is linked to adverse fetal outcomes, mostly microcephaly in newborns, and other clinical aspects such as acute febrile illness and neurologic complications, for example, Guillain-Barré syndrome. One of the most promising strategies to mitigate arbovirus transmission involves releasing
Ae. aegypti
mosquitoes carrying the maternally inherited endosymbiont bacteria
Wolbachia pipientis
. The presence of
Wolbachia
is associated with a reduced susceptibility to arboviruses and a fitness cost in mosquito life-history traits such as fecundity and fertility. However, the mechanisms by which
Wolbachia
influences metabolic pathways leading to differences in egg production remains poorly known. To investigate the impact of coinfections on the reproductive tract of the mosquito, we applied an isobaric labeling-based quantitative proteomic strategy to investigate the influence of
Wolbachia w
Mel and ZIKV infection in
Ae. aegypti
ovaries. To the best of our knowledge, this is the most complete proteome of
Ae. aegypti
ovaries reported so far, with a total of 3913 proteins identified, were also able to quantify 1044
Wolbachia
proteins in complex sample tissue of
Ae. aegypti
ovary. Furthermore, from a total of 480 mosquito proteins modulated in our study, we discuss proteins and pathways altered in
Ae. aegypti
during ZIKV infections,
Wolbachia
infections, coinfection
Wolbachia
/ZIKV, and compared with no infection, focusing on immune and reproductive aspects of
Ae. aegypti
. The modified aspects mainly were related to the immune priming enhancement by
Wolbachia
presence and the modulation of the Juvenile Hormone pathway caused by both microorganism’s infection. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Rushika Perera, Colorado State University, United States; Victoria Pando-Robles, National Institute of Public Health, Mexico These authors have contributed equally to this work This article was submitted to Virus and Host, a section of the journal Frontiers in Cellular and Infection Microbiology Edited by: Henry Puerta-Guardo, Universidad Autónoma de Yucatán, Mexico |
ISSN: | 2235-2988 2235-2988 |
DOI: | 10.3389/fcimb.2022.900608 |