Prevalence of a Streptococcal Inhibitor of a Complement-Mediated Cell lysis-like Gene (sicG) in Streptococcus Dysgalactiae subsp. Equisimilis

• Published in: Current microbiology Vol. 62; no. 3; pp. 884 - 887
• Main Authors: Minami, Masaaki, Ichikawa, Mariko, Matsui, Hideyuki, Hata, Nanako, Wakiyama, Naoki, Matsumoto, Masakado, Ohta, Michio, Hasegawa, Tadao
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.03.2011; Springer-Verlag; Springer Nature B.V
• Subjects: Amino Acid Sequence; Antigens, Bacterial - genetics; Bacterial Outer Membrane Proteins - genetics; Bacterial proteins; Bacterial Proteins - genetics; Biomedical and Life Sciences; Biotechnology; Carrier Proteins - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Genotype & phenotype; Humans; Life Sciences; Microbiology; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA; Streptococcus - genetics; Streptococcus dysgalactiae; Streptococcus infections; Streptococcus pyogenes; Virulence Factors - genetics; Invasive Disease; Streptococcus Dysgalactiae; Terminal Complement Complex; Southern Blotting Analysis; Streptococcal Strain
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-010-9798-8

Streptococcus dysgalactiae subsp. equisimilis isolates (n = 110) were analyzed by PCR to determine whether the gene encoding SICG, a homolog of Streptococcus pyogenes SIC, was present. Nineteen strains (17%) had this gene of which 11 (55%) were isolated from patients with invasive disease. All 19 strains possessed group G carbohydrate. Molecular characterization of emm type revealed that the majority of emm sequences were stG643 and stG2078. Only the N-terminal sequence of SICG was similar to that of SIC in S. pyogenes. Although we found no significant relationship between pathogenic severity and sicG possession, further investigation into the mechanism of SICG may elucidate the virulence in S. dysgalactiae subsp. equisimilis infection.