A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples

• Published in: Biosensors & bioelectronics Vol. 87; pp. 823 - 831
• Main Authors: Soares, R.R.G., Santos, D.R., Chu, V., Azevedo, A.M., Aires-Barros, M.R., Conde, J.P.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.01.2017
• Subjects: aflatoxin B1; Aflatoxin B1 - analysis; Aflatoxins; antibodies; Arrays; Biosensing Techniques - instrumentation; biosensors; chemiluminescence; deoxynivalenol; Devices; Equipment Design; European Union; feedstocks; Food Contamination - analysis; Horseradish Peroxidase - chemistry; Immunoassay; Immunoassay - instrumentation; immunoassays; Immunoconjugates - chemistry; Lab-On-A-Chip Devices; Laminar; laminar flow; Limit of Detection; Luminescent Measurements - instrumentation; Magnets; Microfluidics; Multiplexing; Mycotoxins; ochratoxin A; Ochratoxins - analysis; Panels; Patterning; Photoconductors; screening; silicon; Trichothecenes - analysis; Multiplexing; Photoconductors; Patterning; Magnets; Mycotoxins; Laminar
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.09.041

For a point-of-use analytical device to be successful in real-world applications, it needs to be rapid, simple to operate and, ideally, able to multiplex the detection of several analytes and samples. Mycotoxin detection in food and feedstock in particular has become increasingly relevant as these toxins, such as ochratoxin A (OTA), aflatoxin B1 (AFB1) and deoxynivalenol (DON), are subject to strict regulations and recommendations in the European Union. A novel, simple, negative pressure-driven device with manually operated magnetic valves was developed and the simultaneous immunodetection of these three mycotoxins was demonstrated via the laminar flow patterning of probes in an area of ≈0.12mm2 and subsequent chemiluminescence generation via HRP-labeled antibodies. The three mycotoxins were detected in less than 20min at concentrations of 100ng/mL for OTA and DON and 3ng/mL for AFB1, spiked in a sample under analysis and simultaneously compared to a toxin-free reference and a standard contaminated with critical target concentrations. The on-chip optical detection was performed in a single acquisition step by integrating a microfabricated array of 25×25µm2 hydrogenated amorphous silicon (a-Si:H) photosensors below the microfluidic chip. The device presented in this work is simple and effective for point-of-use multiplexing of immunoassays and was applied in this work to the screening of mycotoxins. [Display omitted] •A multiplexing microfluidic device to analyze 3 analytes in 3 samples was developed.•The device is simple to operate, utilizing negative pressure and magnetic valves.•The signal is generated in 9 biosensing regions via chemiluminescent immunoassays.•The device was integrated with a 0.12mm2 array of nine 25×25µm photoconductors.•Three mycotoxins were detected simultaneously at relevant regulatory limits.