Effects of Lycium barbarum aqueous and ethanol extracts on high-fat-diet induced oxidative stress in rat liver tissue

• Published in: Molecules (Basel, Switzerland) Vol. 16; no. 11; pp. 9116 - 9128
• Main Authors: Cui, BoKang, Liu, Su, Lin, XiaoJun, Wang, Jun, Li, ShuHong, Wang, QiBo, Li, ShengPing
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.11.2011; MDPI
• Subjects: Alanine Transaminase - blood; Alkaline Phosphatase - blood; Animals; antioxidant; Antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Aspartate Aminotransferases - blood; AST; Cholesterol; Diet; Diet, High-Fat - adverse effects; Enzymes; Ethanol; Ethanol - chemistry; hepatoprotection; Homeostasis; Industrialized nations; Insulin resistance; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Liver - drug effects; Liver - enzymology; Liver cancer; Liver cirrhosis; Liver diseases; Lycium - chemistry; Lycium barbarum; Male; Metabolic syndrome; Oxidative stress; Oxidative Stress - drug effects; Phosphatase; Plant Extracts - chemistry; Plant Extracts - pharmacology; Protective Agents - chemistry; Protective Agents - pharmacology; Random Allocation; rat; Rats; Rats, Wistar; Water - chemistry; China; Guangdong China
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules16119116

This study evaluated the protective effects of aqueous extract of Lycium barbarum (LBAE) and ethanol extract of Lycium barbarum (LBEE) on blood lipid levels, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities and liver tissue antioxidant enzyme activities in rats fed a high fat diet (HF). The rats were randomly divided into seven groups of ten rats each and fed a different diet for eight weeks as follows: One group (NC group) was fed a standard diet, one group was fed a high-fat diet (HF group), one group was fed a high-fat diet and orally fed with 20 mg/kg b.w. simvastatin (HF + simvastatin group), and the other group was fed the high fat diet and orally fed with 50 mg/kg b.w. or 100 mg/kg b.w. LBAE (HF + LBAE), or 50 mg/kg b.w. or 100 mg/kg b.w. LBEE (HF + LBEE), respectively. After eight weeks, the HF diet caused deleterious metabolic effects. Rats fed the HF diet alone showed increased hepatocellular enzyme activities in plasma, a significant decline in antioxidant enzyme activities, and elevated liver lipid peroxidation indices. LBAE and LBEE administration significantly reduced liver damage and oxidative changes, and brought back the antioxidants and lipids towards normal levels. These data suggest that these antioxidants protect against toxicity parameters in HF rats.