Structural optimization of siRNA conjugates for albumin binding achieves effective MCL1-directed cancer therapy

• Published in: Nature communications Vol. 15; no. 1; p. 1581
• Main Authors: Hoogenboezem, Ella N., Patel, Shrusti S., Lo, Justin H., Cavnar, Ashley B., Babb, Lauren M., Francini, Nora, Gbur, Eva F., Patil, Prarthana, Colazo, Juan M., Michell, Danielle L., Sanchez, Violeta M., McCune, Joshua T., Ma, Jinqi, DeJulius, Carlisle R., Lee, Linus H., Rosch, Jonah C., Allen, Ryan M., Stokes, Larry D., Hill, Jordan L., Vickers, Kasey C., Cook, Rebecca S., Duvall, Craig L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.02.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 13/89; 38; 631/154/152; 631/61/51/2053; 631/67/1347; Albumin; Apoptosis; Binding; Breast cancer; Cancer therapies; Conjugates; Humanities and Social Sciences; Hydrophobicity; Lipids; Mcl-1 protein; Molecular structure; multidisciplinary; Oncogenes; Pharmacokinetics; Science; Science (multidisciplinary); Self-assembly; Serum albumin; Silence; siRNA; Structure-function relationships; Tumors
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-45609-0

The high potential of siRNAs to silence oncogenic drivers remains largely untapped due to the challenges of tumor cell delivery. Here, divalent lipid-conjugated siRNAs are optimized for in situ binding to albumin to improve pharmacokinetics and tumor delivery. Systematic variation of the siRNA conjugate structure reveals that the location of the linker branching site dictates tendency toward albumin association versus self-assembly, while the lipid hydrophobicity and reversibility of albumin binding also contribute to siRNA intracellular delivery. The lead structure increases tumor siRNA accumulation 12-fold in orthotopic triple negative breast cancer (TNBC) tumors over the parent siRNA. This structure achieves approximately 80% silencing of the anti-apoptotic oncogene MCL1 and yields better survival outcomes in three TNBC models than an MCL-1 small molecule inhibitor. These studies provide new structure-function insights on siRNA-lipid conjugate structures that are intravenously injected, associate in situ with serum albumin, and improve pharmacokinetics and tumor treatment efficacy. Limited tumor cell delivery is a major challenge for the efficacious delivery of siRNAs to silence traditionally undruggable oncogenes. Here the authors optimize siRNAs for in situ binding to albumin through C18 lipid modifications and show the application of the lead conjugate structure for targeting MCL1 in orthotopic breast tumors in mice.