IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay

• Published in: Journal of neuroimmunology Vol. 301; pp. 16 - 22
• Main Authors: Kadoya, Masato, Kaida, Kenichi, Koike, Haruki, Takazaki, Hiroshi, Ogata, Hidenori, Moriguchi, Kota, Shimizu, Jun, Nagata, Eiichiro, Takizawa, Shunya, Chiba, Atsuro, Yamasaki, Ryo, Kira, Jun-ichi, Sobue, Gen, Ikewaki, Katsunori
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.12.2016
• Subjects: Adolescent; Adult; Aged; Allergy and Immunology; Animals; Antibody; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - immunology; Cell Adhesion Molecules, Neuronal - metabolism; Chronic inflammatory demyelinating polyradiculoneuropathy; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; HEK293 Cells; Humans; IgG4; Immunoglobulin G - blood; Male; Middle Aged; Nerve Growth Factors - genetics; Nerve Growth Factors - immunology; Neurofascin155; Neurology; Polyradiculoneuropathy, Chronic Inflammatory Demyelinating - blood; Polyradiculoneuropathy, Chronic Inflammatory Demyelinating - immunology; Polyradiculoneuropathy, Chronic Inflammatory Demyelinating - pathology; Rats; Sciatic Nerve - metabolism; Statistics, Nonparametric; Transfection; Young Adult; IgG4; Neurofascin155; Chronic inflammatory demyelinating polyradiculoneuropathy; Antibody; Enzyme-linked immunosorbent assay
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2016.10.013

We aimed to validate the diagnostic utility of enzyme-linked immunosorbent assay (ELISA) for the detection of anti-neurofascin (NF) 155 antibody in 191 patients with chronic inflammatory demyelinating polyneuropathy (CIDP). Human NF155-based ELISA clearly distinguished between anti-NF155 antibody-positive and -negative sera. Fifteen CIDP patients (8%) were IgG4 anti-human NF155 antibody-positive, which were confirmed by western blot, cell-based assay and immunohistochemical study. None of disease controls or healthy subjects had positive results. Clinical presentation of IgG4 anti-NF155 antibody-positive patients was consistent with those in previous reports. This ELISA combined with determination of the IgG4 subclass is useful in screening for anti-NF155 antibodies. [Display omitted] •Anti-Neurofascin155 antibody was measured in CIDP patients by multiple methods.•The diagnostic utility of human NF155-based ELISA for screening is validated.•Western blot and CBA are useful for confirmation of positive results in ELISA.•Development of the optimal treatment will require gathering of more information.