Antirepressor specificity is shaped by highly efficient dimerization of the staphylococcal pathogenicity island regulating repressors: Stl repressor dimerization perturbed by dUTPases

• Published in: Scientific reports Vol. 14; no. 1; pp. 1953 - 8
• Main Authors: Nyíri, Kinga, Gál, Enikő, Laczkovich, Máté, Vértessy, Beáta G.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.01.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/45/612/1229; 631/45/612/1256; Biosensors; Data analysis; Dimerization; E coli; Enzymes; Equilibrium; Genomic Islands; Humanities and Social Sciences; Humans; Interferometry; Islands; Life cycles; multidisciplinary; Mutagenesis; Pathogenicity; Pathogenicity islands; Pathogens; Phenols; Polymers; Proteins; Pyrophosphatases; Quantitative analysis; Repressors; Science; Science (multidisciplinary); Sensors; Staphylococcal Infections; Staphylococcus; Staphylococcus aureus
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-51260-y

The excision and replication, thus the life cycle of pathogenicity islands in staphylococci are regulated by Stl master repressors that form strong dimers. It has been recently shown that SaPIbov1-Stl dimers are separated during the activation of the Staphylococcus aureus pathogenicity island (SaPI) transcription via helper phage proteins. To understand the mechanism of this regulation, a quantitative analysis of the dimerization characteristics is required. Due to the highly efficient dimerization process, such an analysis has to involve specific solutions that permit relevant experiments to be performed. In the present work, we focused on two staphylococcal Stls associated with high biomedical interest, namely Stl proteins of Staphylococcus aureus bov1 and Staphylococcus hominis ShoCI794_SEPI pathogenicity islands. Exploiting the interactions of these two Stl proteins with their antirepressor-mimicking interaction partners allowed precise determination of the Stl dimerization constant in the subnanomolar range.