Mechanism of calcitonin gene related peptide against acute pancreatitis in rats by modulating amino acid metabolism based on metabonomics

• Published in: Scientific reports Vol. 15; no. 1; pp. 6686 - 10
• Main Authors: Hu, Jian-Xiong, Chen, Ying-Kai, Chen, Shi-Jun, Lin, Yan-Ya, Chen, Jun-Nian, Xie, Ying, Zhao, Cheng-Fei, Chen, Cun-Rong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.02.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 692/308; 692/420; Acute Disease; Acute pancreatitis; Amino acid; Amino acids; Amino Acids - metabolism; Animals; Calcitonin; Calcitonin gene-related peptide; Calcitonin Gene-Related Peptide - metabolism; Calcitonin Gene-Related Peptide - pharmacology; Carbohydrate metabolism; Carbohydrates; CGRP; Digestive system; Disease Models, Animal; Glutamine; Humanities and Social Sciences; Inflammation; Lipid metabolism; Male; Metabolic pathways; Metabolism; Metabolites; Metabolome; Metabolomics; Metabolomics - methods; multidisciplinary; Ornithine; Pancreas - metabolism; Pancreas - pathology; Pancreatitis; Pancreatitis - drug therapy; Pancreatitis - metabolism; Pancreatitis - pathology; Peptide Fragments - pharmacology; Peptides; Proline; Rats; Rats, Sprague-Dawley; Science; Science (multidisciplinary); Valine; Metabolomics; Acute pancreatitis; Amino acid; CGRP
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-025-87707-z

To study the mechanism of calcitonin gene related peptide(CGRP) protecting acute pancreatitis based on metabolomics. 24 adult male rats were randomly divided into control group (Con), acute pancreatitis model group (AP), CGRP treatment group (CGRP + AP, abbreviated as CGRP) and CGRP antagonist(CGRP(8–37)) pretreatment group (preCGRP(8–37) + AP, abbreviated as CGRP37), with 6 rats in each group. After different interventions, pancreases of rats in each group were collected for pathological analysis, and serum was collected for metabolomics analysis. Pathological examination of the pancreas suggested that the inflammation of pancreatitis in AP group was significant, the inflammation of pancreatitis in CGRP group was significantly reduced, and the pancreatitis in CGRP37 group was aggravated. Metabolomics of rat serum suggested that the differences in metabolites in each group were mainly related to amino acid metabolism, coenzyme/vitamin metabolism, carbohydrate metabolism, lipid metabolism, digestive system and other metabolic pathways. According to the trend of metabolite changes, we found 6 differential metabolites that were significantly correlated with CGRP intervention, including L-Valine, 5-Aminopentanoic acid, 4-oxo-L-proline, L-glutamine, L-proline, and Ornithine, all of which were related to amino acid metabolism. CGRP can effectively protect acute pancreatitis, possibly by regulating amino acid metabolism to alleviate acute pancreatitis.