Recent advances in biosensors based on enzyme inhibition

• Published in: Biosensors & bioelectronics Vol. 76; pp. 180 - 194
• Main Authors: Amine, A., Arduini, F., Moscone, D., Palleschi, G.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.02.2016
• Subjects: Analytical applications; Analytical chemistry; Biosensing Techniques - methods; Biosensor; Biosensors; Cost analysis; Devices; Electrochemistry; Environmental Monitoring; Environmental Pollutants - isolation & purification; Enzyme inhibition; Enzyme Inhibitors - isolation & purification; Enzymes; Enzymes, Immobilized - chemistry; Humans; Inhibition; Inhibitors; Nanomaterials; Enzyme inhibition; Nanomaterials; Analytical applications; Biosensor
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.07.010

Enzyme inhibitors like drugs and pollutants are closely correlated to human and environmental health, thus their monitoring is of paramount importance in analytical chemistry. Enzymatic biosensors represent cost-effective, miniaturized and easy to use devices; particularly biosensors based on enzyme inhibition are useful analytical tools for fast screening and monitoring of inhibitors. The present review will highlight the research carried out in the last 9 years (2006–2014) on biosensors based on enzyme inhibition. We underpin the recent advances focused on the investigation in new theoretical approachs and in the evaluation of biosensor performances for reversible and irreversible inhibitors. The use of nanomaterials and microfluidic systems as well as the applications of the various biosensors in real samples is critically reviewed, demonstrating that such biosensors allow the development of useful devices for a fast and reliable alarm system. •Novel theoretical approach of enzyme inhibition.•Analytical performances of biosensors based on enzyme inhibition.•The use of nanomaterials and microfluidic in inhibitive biosensors.