Mass spectrometric characterization of human serum albumin dimer: A new potential biomarker in chronic liver diseases

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 112; pp. 169 - 175
• Main Authors: Naldi, Marina, Baldassarre, Maurizio, Nati, Marina, Laggetta, Maristella, Giannone, Ferdinando Antonino, Domenicali, Marco, Bernardi, Mauro, Caraceni, Paolo, Bertucci, Carlo
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 10.08.2015
• Subjects: Albumin dimers; Biomarkers; Biomarkers - blood; Biomarkers - chemistry; Chronic Disease; Female; Human serum albumin isoforms; Humans; Liver Cirrhosis - blood; Male; Mass spectrometry; Middle Aged; Oxidative Stress - physiology; Post-translational modifications; Protein Isoforms - blood; Protein Isoforms - chemistry; Protein Multimerization; Serum Albumin - biosynthesis; Serum Albumin - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Biomarkers; Post-translational modifications; Mass spectrometry; Albumin dimers; Human serum albumin isoforms
• ISSN: 0731-7085; 1873-264X; 1873-264X
• DOI: 10.1016/j.jpba.2014.12.001

•HSA isoforms are identified and quantified by LC-ESI-Q-TOF and MALDI-TOF.•HSA dimeric form results higher in plasma samples from cirrhotic patients.•The dimerization site is proved to be at Cys-34 of two albumin molecules.•These isoforms represent promising biomarkers for liver disease. Human serum albumin (HSA) undergoes several structural alterations affecting its properties in pro-oxidant and pro-inflammatory environments, as it occurs during liver cirrhosis. These modifications include the formation of albumin dimers. Although HSA dimers were reported to be an oxidative stress biomarker, to date nothing is known about their role in liver cirrhosis and related complications. Additionally, no high sensitive analytical method was available for HSA dimers assessment in clinical settings. Thus the HSA dimeric form in human plasma was characterized by mass spectrometry using liquid chromatography tandem mass spectrometry (LC-ESI-Q-TOF) and matrix assisted laser desorption time of flight (MALDI-TOF) techniques. N-terminal and C-terminal truncated HSA, as well as the native HSA, undergo dimerization by binding another HSA molecule. This study demonstrated the presence of both homo- and hetero-dimeric forms of HSA. The dimerization site was proved to be at Cys-34, forming a disulphide bridge between two albumin molecules, as determined by LC–MS analysis after tryptic digestion. Interestingly, when plasma samples from cirrhotic subjects were analysed, the dimer/monomer ratio resulted significantly increased when compared to that of healthy subjects. These isoforms could represent promising biomarkers for liver disease. Additionally, this analytical approach leads to the relative quantification of the residual native HSA, with fully preserved structural integrity.