Single-shot full-field reflection phase microscopy

We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-s...

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Bibliographic Details
Published inOptics express Vol. 19; no. 8; pp. 7587 - 7595
Main Authors Yaqoob, Zahid, Yamauchi, Toyohiko, Choi, Wonshik, Fu, Dan, Dasari, Ramachandra R, Feld, Michael S
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 11.04.2011
Subjects
Algorithms
Cell Membrane - metabolism
Equipment Design
HeLa Cells
Humans
Interferometry - instrumentation
Interferometry - methods
Light
Microscopy, Phase-Contrast - instrumentation
Microscopy, Phase-Contrast - methods
Models, Statistical
Motion
Optics and Photonics
Signal Processing, Computer-Assisted
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Summary:We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.
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ISSN:1094-4087
1094-4087
DOI:10.1364/oe.19.007587