A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro

• Published in: Journal of materials science. Materials in medicine Vol. 34; no. 7; p. 37
• Main Authors: Mao, Yong, John, Nikita, Protzman, Nicole M., Long, Desiree, Sivalenka, Raja, Azimi, Shamshad, Mirabile, Brandon, Pouliot, Robert, Gosiewska, Anna, Hariri, Robert J., Brigido, Stephen A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 24.07.2023; Springer Nature B.V; Springer
• Subjects: Amniotic membrane; Biological activity; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Cell migration; Ceramics; Chemistry and Materials Science; Collagen; Composites; Cytokines; Dehydration; Design; Glass; Growth factors; Inflammation; Inflammatory response; Materials Science; Mechanical properties; Membranes; Natural Materials; Phosphates; Polymer Sciences; Product design; Regenerative Medicine/Tissue Engineering; Scaffolds; Surfaces and Interfaces; Tendons; Tensile strength; Thin Films; Tissue engineering; Tissue Engineering Constructs and Cell Substrates; Tumor necrosis factor-TNF
• ISSN: 1573-4838; 0957-4530; 1573-4838
• DOI: 10.1007/s10856-023-06740-4

Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity. Graphical Abstract