hShroom1 links a membrane bound protein to the actin cytoskeleton

• Published in: Cellular and molecular life sciences : CMLS Vol. 66; no. 4; pp. 681 - 696
• Main Authors: Dye, D. E, Karlen, S, Rohrbach, B, Staub, O, Braathen, L. R, Eidne, K. A, Coombe, D. R
• Format: Journal Article
• Language: English
• Published: Basel Basel : Birkhäuser-Verlag 01.02.2009; Birkhäuser-Verlag; Springer Nature B.V
• Subjects: Actins - metabolism; Alternative Splicing; Amino Acid Sequence; Animals; Apx; Biochemistry; Biomedical and Life Sciences; Biomedicine; CD146; CD146 Antigen - genetics; CD146 Antigen - metabolism; Cell adhesion & migration; Cell Biology; Cell Line; Cytoskeleton - metabolism; Humans; Life Sciences; MCAM; melanoma; Melanoma - genetics; Melanoma - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Membranes; Microfilament Proteins - genetics; Microfilament Proteins - metabolism; Molecular biology; Molecular Sequence Data; Protein Isoforms - genetics; Protein Isoforms - metabolism; Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Research Article; Shroom; Skin cancer; Two-Hybrid System Techniques; Apx; Shroom; melanoma; CD146; MCAM
• ISSN: 1420-682X; 1420-9071
• DOI: 10.1007/s00018-009-8645-1

hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with β-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton.