A New Method of Extracting Polygonatum sibiricum Polysaccharide with Antioxidant Function: Ultrasound-Assisted Extraction-Deep Eutectic Solvents Method

• Published in: Foods Vol. 12; no. 18; p. 3438
• Main Authors: Sun, Chaoqun, Wang, Guodong, Sun, Jing, Yin, Jiyong, Huang, Jian, Li, Zizi, Mu, Di, He, Menglu, Liu, Tingting, Cheng, Jiali, Du, Hanchen, Chen, Yan, Qu, Wenjie
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 15.09.2023; MDPI
• Subjects: antioxidant activity; antioxidant function; Antioxidants; Biological activity; Dietary supplements; Efficiency; Enzymes; Ethanol; Food; Food industry; Food science; Functional foods & nutraceuticals; Hemodialysis; Industrial production; Methods; Optimization; Phenols; Polygonatum sibiricum; Polygonatum sibiricum Polysaccharide (PsP); Polysaccharides; response surface method (RSM); Response surface methodology; Solvents; Sulfuric acid; temperature; Ultrasonic imaging; ultrasonic treatment; ultrasonics; Ultrasound; ultrasound-assisted extraction-deep eutectic solvents (UAE-DESS); Beijing China; United States > US; China; Germany
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12183438

Polygonatum sibiricum Polysaccharide (PsP) with antioxidant function is the main active component of Polygonatum sibiricum (P.sibiricum). The currently poor extraction yield and extraction methods of PsP cannot meet the application of that in food industrial production. In this research, an ultrasound-assisted extraction-deep eutectic solvents (UAE-DESs) method, which has never been used in the PsP industry, was first used to extract PsP. The extraction conditions were optimized by the response surface method (RSM). Both the extraction yield and antioxidant function were simultaneously considered during the optimization process. The indicators of PsP’s level and antioxidant activity in vitro were used to present the extraction yield of the UAE-DESs method, the purity, and the antioxidant effect of PsP. Under the optimal conditions, which included a liquid–solid ratio of 26:1 (mL:g), extraction temperature of 80 °C, ultrasonic time of 51 min, and ultrasonic power of 82 W, the PsP extraction yield could reach (43.61 ± 0.09)%, which was obviously higher than single DESs (33.81%) and UAE (5.83%), respectively, and the PsP appeared favorably antioxidant function. This research proposed an efficient extraction method for PsP, filled the basic research gap, and further improved the development of PsP as a dietary supplement with antioxidant function in the food industry.