Down-regulation of Treg cells and up-regulation of TH1/TH2 cytokine ratio were induced by polysaccharide from Radix Glycyrrhizae in H22 hepatocarcinoma bearing mice

• Published in: Molecules (Basel, Switzerland) Vol. 16; no. 10; pp. 8343 - 8352
• Main Authors: He, Xiaojuan, Li, Xiaobing, Liu, Biao, Xu, Li, Zhao, Hongyan, Lu, Aiping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.09.2011; MDPI
• Subjects: Animals; Antineoplastic Agents, Phytogenic - pharmacology; Carcinoma, Hepatocellular - drug therapy; Carcinoma, Hepatocellular - immunology; CD4+CD25+ regulatory T cells; Cell Proliferation - drug effects; Cytokines; Cytokines - metabolism; Down-Regulation; Drugs, Chinese Herbal - pharmacology; Female; Forkhead Transcription Factors - biosynthesis; Glycyrrhiza; Interleukin-10 - biosynthesis; Interleukin-12 - biosynthesis; Liver Neoplasms - drug therapy; Liver Neoplasms - immunology; Lymphatic system; Medicine; Mice; Mice, Inbred BALB C; Nitric Oxide - biosynthesis; Nitric Oxide Synthase - biosynthesis; Oxidative Stress - drug effects; Polysaccharides - pharmacology; Radix Glycyrrhizae polysaccharide; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Spleen; T-Lymphocytes, Regulatory - drug effects; T-Lymphocytes, Regulatory - immunology; Th1 Cells - drug effects; Th1 Cells - immunology; Th1-Th2 Balance - drug effects; Th1/Th2 cytokines; Th2 Cells - drug effects; Th2 Cells - immunology; Transforming Growth Factor beta - biosynthesis; tumor; Beijing China; China
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules16108343

Radix Glycyrrhizae polysaccharide (GP) possesses multiple pharmacological activities. However, the effect of GP on CD4+CD25+ regulatory T (Treg) cells has not been elucidated. This study aimed to investigate the effects of GP on Treg cells and Th1/Th2 cytokines in H22 hepatocarcinoma tumor-bearing mice. The results demonstrated that GP inhibits tumor progression. In the lymph nodes of the tumor microenvironment and spleen, the proportion of Treg cells was significantly higher in the tumor-bearing mice. GP administration down-regulated the population of Treg cells (P < 0.01) and decreased lymph node Foxp3 and IL-10 mRNA expression (P < 0.01). In addition, GP treatment decreased IL-10 and TGF-β level (P < 0.01) and increased IL-2 and IL-12p70 level in serum (P < 0.01). In conclusion, GP reduced the proportion of Treg cells and Foxp3 lowered expression in Treg cells, and up-regulated Th1/Th2 cytokine ratio in serum in the tumor bearing mice, which might partially cause the inhibition of tumor growth.