Expression changes of cytoskeletal associated proteins in proteomic profiling of neuroblastoma cells infected with different strains of rabies virus

• Published in: Journal of medical virology Vol. 85; no. 2; pp. 336 - 347
• Main Authors: Zandi, Fatemeh, Eslami, Naser, Torkashvand, Fatemeh, Fayaz, Ahmad, Khalaj, Vahid, Vaziri, Behrouz
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.02.2013; Wiley; Wiley Subscription Services, Inc; Wiley-Blackwell
• Subjects: Actin; Animals; Biological and medical sciences; Cell Line; cytoskeletal changes; Cytoskeletal Proteins - analysis; Cytoskeleton; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Gene Expression; Host-Pathogen Interactions; Human viral diseases; Immunology; Infectious diseases; Life Sciences; Mass Spectrometry; Medical sciences; Mice; Microbiology; Miscellaneous; Neurons - chemistry; Neurons - virology; pathogenesis; Pathology; Proteomics; Rabies; Rabies virus; Rabies virus - growth & development; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Viral diseases; Virology; Nervous system diseases; Pathogenesis; Malignant tumor; Neuroblastoma; Rabies virus; Protein; Strain; Virus; Mononegavirales; cytoskeletal changes; Rhabdoviridae; Proteomics; Lyssavirus; Infected cell; Autonomic neuropathy; Cancer; Rabies Virus; Neuroblastoma Cells; pathogenesis; Cytoskeletal; Proteomic
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.23458

Rabies virus invades the nervous system, induces neuronal dysfunction and causes death of the host. The disruption of the cytoskeletal integrity and synaptic structures of the neurons by rabies virus has been postulated as a possible basis for neuronal dysfunction. In the present study, a two‐dimensional electrophoresis/mass spectrometry proteomics analysis of neuroblastoma cells revealed a significant effect of a virulent strain of rabies virus on the host cytoskeleton related proteins which was quite different from that of an attenuated strain. Vimentin, actin cytoplasmic 1 isoform, profilin I, and Rho‐GDP dissociation inhibitor were host cell cytoskeletal related proteins changed by the virulent strain. The proteomics data indicated that the virulent strain of rabies virus induces significant expression changes in the vimentin and actin cytoskeleton networks of neurons which could be a strong clue for the relation of cytoskeletal integrity distraction and rabies virus pathogenesis. In addition, the expression alteration of other host proteins, particularly some structural and regulatory proteins may have potential roles in rabies virus pathogenesis. J. Med. Virol. 85:336–347, 2013. © 2012 Wiley Periodicals, Inc.