Neurochemical characteristics of a novel dorsal root ganglion X neuroblastoma hybrid cell line, F-11

• Published in: Journal of neurochemistry Vol. 48; no. 5; p. 1624
• Main Authors: Francel, P C, Harris, K, Smith, M, Fishman, M C, Dawson, G, Miller, R J
• Format: Journal Article
• Language: English
• Published: England 01.05.1987
• Subjects: Animals; Cell Line; Ganglia, Spinal - cytology; Ganglia, Spinal - metabolism; Ion Channels - metabolism; Ion Channels - physiology; Neurons, Afferent - metabolism; Receptors, Bradykinin; Receptors, Neurotransmitter - metabolism; Receptors, Opioid - metabolism; Substance P - metabolism
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1111/j.1471-4159.1987.tb05711.x

We investigated the properties of the novel dorsal root ganglion (DRG) hybrid cell line F-11 to see how closely these cells resembled normal DRG cells. Under normal growth conditions, F-11 cells appeared to contain several short neurite-like processes. However, these cells could also be grown under conditions in which they showed a much more extensive neuronal morphology, exhibiting many long neurites. Several differentiated features of DRG cells were present on F-11 cells. These included the presence of delta-opioid receptors, receptors for prostaglandins and bradykinin, and dihydropyridine-sensitive calcium channels. F-11 cells also synthesized and released a substance P-like compound, as determined by immunoreactivity. Both the number of bradykinin receptors and the voltage-sensitive calcium influx increased on cell differentiation. Opioid agonists (delta-specificity) were found to decrease cyclic AMP levels in F-11 cells in a naloxone- and pertussis toxin-reversible fashion. Bradykinin stimulated the synthesis of inositol-1,4-bisphosphate and inositol-1,4,5-trisphosphate. Ca2+ channel agonists stimulated voltage-sensitive Ca2+ influx in a dose-dependent, stereospecific manner, whereas Ca2+ channel antagonists inhibited Ca2+ influx. F-11 cells should, therefore, prove useful as models for authentic DRG neurons.