Immunostimulating factor isolated from actinobacillus actinomycetemcomitans stimulates monocytes and inflammatory macrophages

• Published in: Microbiology and immunology Vol. 50; no. 7; p. 535
• Main Authors: Jo, Wol-Soon, Yee, Sung-Tae, Yoon, Sik, Nam, Byung-Huouk, Do, Eunju, Jung, Beom-Seok, Jeong, Soo-Jin, Hong, Sook-Hee, Yoo, Young-Hyun, Kang, Chi-Dug, Lim, Young-Jin, Jeong, Min-Ho, Lee, Jae-Dong
• Format: Journal Article
• Language: English
• Published: Australia 01.01.2006
• Subjects: Adjuvants, Immunologic - isolation & purification; Adjuvants, Immunologic - pharmacology; Aggregatibacter actinomycetemcomitans - chemistry; Aggregatibacter actinomycetemcomitans - immunology; Animals; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Cell Line, Tumor; Dendritic Cells - drug effects; Dendritic Cells - immunology; Drug Synergism; Female; Humans; Lipopolysaccharides - pharmacology; Macrophages - drug effects; Macrophages - immunology; Mice; Mice, Inbred BALB C; Monocytes - drug effects; Monocytes - immunology; Nitric Oxide - metabolism; Spleen - cytology; Spleen - drug effects; Spleen - immunology
• ISSN: 0385-5600
• DOI: 10.1111/j.1348-0421.2006.tb03823.x

Immunostimulating factor (ISTF) isolated from Actinobacillus actinomycetemcomitans which has been described previously, is distinct from lipopolysaccharide and induces proliferation of B cells. This study was undertaken to investigate whether ISTF might enhance the stimulation of other immune cells. Immunohistochemically, ISTF exhibited a profound stimulating effect on macrophages and dendritic cells as well as B cells in the spleen of BALB/c mice. ISTF was also recognized for its capacity to induce direct activation of mouse macrophages to produce IL-6, TNF-alpha, and NO and MHC class II expression. Therefore, it is postulated that ISTF stimulates macrophages and possibly other cells to produce a wide variety of proinflammatory mediators, which may be involved in the chronicity and tissue destruction of periodontal disease.