Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro

• Published in: Journal of oral pathology & medicine Vol. 42; no. 10; pp. 799 - 806
• Main Authors: Tsujigiwa, Hidetsugu, Katase, Naoki, Lefeuvre, Mathieu, Yamachika, Eiki, Tamamura, Ryo, Ito, Satoshi, Takebe, Yuichiro, Matsuda, Hiroyuki, Nagatsuka, Hitoshi
• Format: Journal Article
• Language: English
• Published: Frederiksberg Blackwell Publishing Ltd 01.11.2013; Wiley
• Subjects: Alkaline Phosphatase - analysis; Animal models; Animals; Biocompatible Materials - chemistry; Biological and medical sciences; Calcification, Physiologic - physiology; Calcium Phosphates - chemistry; Cell Culture Techniques; Cell Differentiation - physiology; Cell Line; Cells, Cultured; Collagen - biosynthesis; Collagen - chemistry; Culture Media; dental pulp; Dental Pulp - cytology; Dental Pulp - physiology; Dentinogenesis - physiology; Dentistry; Drug Combinations; Extracellular Matrix Proteins - analysis; Female; Fibroblasts - physiology; Laminin - chemistry; Male; matrix formation; Medical sciences; Mice; Mice, SCID; Odontoblasts - physiology; Odontoblasts - transplantation; osteoblast; Osteogenesis - physiology; Otorhinolaryngology. Stomatology; Phosphoproteins - analysis; Proteoglycans - chemistry; Rats; Rats, Transgenic; Sialoglycoproteins - analysis; stem cell-like cell; Subcutaneous Tissue - surgery; Tissue Scaffolds - chemistry; stem cell―like cell; In vivo; matrix formation; Stomatology; Stem cell; Osteoblast; ENT; Odontoblast; In vitro; Dental pulp; dental pulp; stem cell-like cell; osteoblast
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/jop.12080

Tooth tissue engineering offers very attractive perspectives for elaboration of regenerative treatments, which enables to cure tooth loss and restore quality of life of the patients. To elaborate such treatment, isolation and culture of dental pulp cell must be achieved as a key element. In this article, we report the establishment of a stable cell line from GFP transgenic rat dental pulp, named TGC (Tooth Matrix‐forming, GFP Rat‐derived Cell). TGCs have exhibited odontoblastic feature both in vitro and in vivo. In vitro, TGC exposed to osteogenic medium demonstrated collagen fiber synthesis with matrix vesicle and mineralization and formed a sheet‐like substrate on the cell culture dish. Increased ALP activity and elevated transcription level of various genes involved in calcification and dentin formation were also observed. In vivo, transplanted TGC in SCID mice with β‐TCP particles formed dentin‐like and pulp‐like structure with lining odontoblast. Notably, even after up to 80 passages, TGCs retain their morphological features and differentiation ability. To our knowledge, this is the first report of a dental pulp–derived cell with such stable odontoblastic characteristics. TGC could be a very useful model for further study on dental pulp cell.