Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes

• Published in: Scientific reports Vol. 8; no. 1; pp. 3120 - 13
• Main Authors: Wang, Wen-Han, Zhang, Jing-Song, Feng, Ting, Deng, Jing, Lin, Chi-Chung, Fan, Hua, Yu, Wen-Juan, Bao, Hai-Ying, Jia, Wei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.02.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 631/250/1619/40/1774; 631/45/72/1205; 82/58; 82/6; Animals; B-Lymphocytes - drug effects; Cell Proliferation - drug effects; China; D-Galactose; Dietary Carbohydrates - analysis; Flammulina - chemistry; Fruit bodies; Fruiting Bodies, Fungal - chemistry; Fucose; Functional foods & nutraceuticals; Galactose; Glucose; Humanities and Social Sciences; Immunoglobulin G; Immunoglobulin M; Immunomodulation; Immunomodulation - drug effects; Lymphocytes; Lymphocytes - drug effects; Lymphocytes B; Magnetic Resonance Spectroscopy - methods; Mannose; Methylation; Mice; Mice, Inbred C57BL; Molecular Weight; Monosaccharides - analysis; multidisciplinary; NMR; Nuclear magnetic resonance; Polysaccharides - chemistry; Rodents; Science; Science (multidisciplinary); Spectroscopy, Fourier Transform Infrared - methods; Spleen; Spleen - cytology; China
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-21375-0

A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes . Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 10 4  Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5. In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner. The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment. So FVPB2 has potential to be a new important immunomodulatory nutraceutical.