Interfacial electronic effects in functional biolayers integrated into organic field-effect transistors

Biosystems integration into an organic field-effect transistor (OFET) structure is achieved by spin coating phospholipid or protein layers between the gate dielectric and the organic semiconductor. An architecture directly interfacing supported biological layers to the OFET channel is proposed and,...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 109; no. 17; pp. 6429 - 6434
Main Authors Angione, Maria Daniela, Cotrone, Serafina, Magliulo, Maria, Mallardi, Antonia, Altamura, Davide, Giannini, Cinzia, Cioffi, Nicola, Sabbatini, Luigia, Fratini, Emiliano, Baglioni, Piero, Scamarcio, Gaetano, Palazzo, Gerardo, Torsi, Luisa
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 24.04.2012
National Acad Sciences
Subjects
Anesthesia
Anesthetics
Bacteriorhodopsins
biosensors
biotin
Coatings
Dielectric materials
Electric current
lipid bilayers
Lipids
Membranes
Molecules
Organic semiconductors
phospholipids
Physical Sciences
Proteins
Protons
Sensors
streptavidin
Transistors
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Summary:Biosystems integration into an organic field-effect transistor (OFET) structure is achieved by spin coating phospholipid or protein layers between the gate dielectric and the organic semiconductor. An architecture directly interfacing supported biological layers to the OFET channel is proposed and, strikingly, both the electronic properties and the biointerlayer functionality are fully retained. The platform bench tests involved OFETs integrating phospholipids and bacteriorhodopsin exposed to 1–5% anesthetic doses that reveal drug-induced changes in the lipid membrane. This result challenges the current anesthetic action model relying on the so far provided evidence that doses much higher than clinically relevant ones (2.4%) do not alter lipid bilayers’ structure significantly. Furthermore, a streptavidin embedding OFET shows label-free biotin electronic detection at 10 parts-per-trillion concentration level, reaching state-of-the-art fluorescent assay performances. These examples show how the proposed bioelectronic platform, besides resulting in extremely performing biosensors, can open insights into biologically relevant phenomena involving membrane weak interfacial modifications.
Bibliography:http://dx.doi.org/10.1073/pnas.1200549109
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1M.D.A., S.C., and M.M. contributed equally to this work.
Author contributions: G.P. and L.T. designed research; M.D.A., S.C., M.M., A.M., D.A., N.C., E.F., and G.S. performed research; C.G., L.S., and P.B. contributed new reagents/analytic tools; and G.P. and L.T. wrote the paper.
Edited by Federico Capasso, Harvard University, Cambridge, MA, and approved February 22, 2012 (received for review January 11, 2012)
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1200549109