Vaccination of mice with recombinant bacille Calmette-Guérin harboring Rv1357c protects similarly to native BCG [Short communication]

• Published in: The international journal of tuberculosis and lung disease Vol. 16; no. 6; pp. 774 - 776
• Main Authors: Flores-Valdez, M. A., de Jesús Aceves-Sánchez, M., Montero-Pérez, S. A., Sánchez-López, A. D., Gutiérrez-Pabello, J. A., Hernández-Pando, R.
• Format: Journal Article
• Language: English
• Published: Paris, France International Union Against Tuberculosis and Lung Disease 01.06.2012; International Union against Tuberculosis and Lung Disease
• Subjects: Acyltransferases - genetics; Acyltransferases - immunology; Animals; Bacterial diseases; BCG Vaccine - immunology; Biological and medical sciences; C-Di-Gmp; Cell Line; Disease Models, Animal; Human bacterial diseases; Infectious diseases; Macrophages - immunology; Macrophages - microbiology; Medical sciences; Mice; Mice, Inbred BALB C; Mycobacterium bovis; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - growth & development; Mycobacterium tuberculosis - immunology; Phagocytosis; Pneumology; Time Factors; Tuberculosis; Tuberculosis and atypical mycobacterial infections; Tuberculosis, Pulmonary - immunology; Tuberculosis, Pulmonary - microbiology; Tuberculosis, Pulmonary - prevention & control; Vaccination; Vaccine; Vaccines, Synthetic - immunology; c-di-GMP; Vaccination; Rodentia; BCG; Vaccine; Mycobacterial infection; GMP; Infection; Prevention; Immunoprophylaxis; Vertebrata; Mammalia; Tuberculosis; Mouse; Animal; Bacteriosis; Pneumology
• ISSN: 1027-3719; 1815-7920
• DOI: 10.5588/ijtld.11.0735

Despite the availability of a Mycobacterium bovis bacille Calmette Guérin (BCG) vaccine, tuberculosis (TB) remains a global public health problem. In this study, we introduced the c-di-GMP phosphodiesterase gene Rv1357c, implicated in regulating mycobacterial replication within macrophages, into BCG Pasteur, and tested the resulting strain for its capacity to serve as a vaccine against TB in a murine model. Modified BCG was more phagocytosed than its parental strain, but halted bacterial replication, and protected against M. tuberculosis challenge similarly to unmodified BCG.