Western blot analysis of cereal grain prolamins using an antibody to carboxyl-linked indoleacetic acid
A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avene sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanol-soluble protein fraction of the seed. Western...
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Published in | Plant physiology (Bethesda) Vol. 96; no. 4; pp. 1076 - 1078 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Rockville, MD
American Society of Plant Physiologists
01.08.1991
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Subjects | |
Online Access | Get full text |
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Summary: | A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avene sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanol-soluble protein fraction of the seed. Western blotting of commercial zein, the major storage protein of maize, along with physicochemical evidence reported by Leverone et al. ([1991] Plant Physiol, 96: 1070-1075) indicated that IAA is linked with this prolamin. Results suggest that an IAA-prolamin association may be widespread throughout the Poaceae. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.96.4.1076 |