transfer and characterization of resistance to common root rot from Thinopyrum ponticum to wheat

• Published in: Genome Vol. 47; no. 1; pp. 215 - 223
• Main Authors: Li, H, Conner, R.L, Chen, Q, Laroche, A, Graf, R.J, Kuzyk, A.D
• Format: Journal Article
• Language: English
• Published: Ottawa, Canada NRC Research Press 01.02.2004; Canadian Science Publishing NRC Research Press
• Subjects: Agribusiness; Agricultural production; Agricultural research; Ascomycota; Botany; Canada; chromosome number; chromosome translocation; Chromosomes; Cochliobolus sativus; color; Crop diseases; Cultivars; disease resistance; fungal diseases of plants; genes; Genetically altered foods; genomic in situ hybridization; Genomics; Hybridization; Hybridization, Genetic; Immunity, Innate - genetics; In Situ Hybridization; intergeneric hybridization; introgression; Karyotyping; Linear Models; nucleic acid hybridization; Phenotype; plant breeding; Plant Diseases - genetics; Plant resistance; Poaceae - genetics; Prairies; root rot; Roots (Botany); seeds; Soil-borne diseases; Spring wheat; Thinopyrum ponticum; Translocation, Genetic - genetics; Triticum - genetics; Triticum aestivum; Wheat; wild relatives; Winter wheat; Canada
• ISSN: 0831-2796; 1480-3321
• DOI: 10.1139/g03-095

Common root rot, caused by Cochliobolus sativus (Ito and Kurib) Drechs. ex Dastur, is a major soil-borne disease of spring and winter wheat (Triticum aestivum L. em Thell.) on the Canadian prairies. Resistance to common root rot from Thinopyrum ponticum (Podp.) Liu and Wang was transferred into wheat via crossing with Agrotana, a resistant wheat - Th. ponticum partial amphiploid line. Evaluation of common root rot reactions showed that selected advanced lines with blue kernel color derived from a wheat x Agrotana cross expressed more resistance than the susceptible T. aestivum 'Chinese Spring' parent and other susceptible wheat check cultivars. Cytological examination revealed 41 to 44 chromosomes in the advanced lines. Genomic in situ hybridization, using total genomic DNA from Pseudoroegneria strigosa (M. Bieb) A. Love (St genome) as a probe, demonstrated that the blue kernel plants had two pairs of spontaneously translocated J-J(s) and J(s)-J chromosomes derived from the J and J(s) genome of Th. ponticum. The presence of these translocated chromosomes was associated with increased resistance of wheat to common root rot. The lines with blue aleurone color always had a subcentromeric J(s)-J translocated chromosome. The subtelocentric J-J(s) translocated chromosome was not responsible for the blue kernel color. The genomic in situ hybridization analysis on meiosis revealed that the two spontaneous translocations were not reciprocal translocations.