Frame localisation optical projection tomography

We present a tomographic reconstruction algorithm (flOPT), which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift. OPT relies on precise mechanical rotation and is less mechanically stable than large-scale computer...

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Bibliographic Details
Published inScientific reports Vol. 11; no. 1; p. 4551
Main Authors Russell, Craig T., Vallejo Ramirez, Pedro P., Rees, Eric
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 25.02.2021
Nature Publishing Group
Nature Portfolio
Subjects
639/624/1107/328
639/624/1107/510
Algorithms
Drift
Humanities and Social Sciences
Image processing
multidisciplinary
Radon
Science
Science (multidisciplinary)
Tomography
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Summary:We present a tomographic reconstruction algorithm (flOPT), which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift. OPT relies on precise mechanical rotation and is less mechanically stable than large-scale computer tomography (CT) scanning systems, leading to reconstruction artefacts. The algorithm uses multiple (5+) tracked fiducial beads to recover the sample pose and the image rays are then back-projected at each orientation. The quality of the image reconstruction using the proposed algorithm shows an improvement when compared to the Radon transform. Moreover, when adding a systematic spatial and angular mechanical drift, the reconstruction shows a significant improvement over the Radon transform.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-83454-z