New triterpene saponins from Duranta repens Linn. and their cytotoxic activity

• Published in: Molecules (Basel, Switzerland) Vol. 14; no. 5; pp. 1952 - 1965
• Main Authors: Ahmed, Wafaa S, Mohamed, Mona A, El-Dib, Rabab A, Hamed, Manal M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.05.2009; Molecular Diversity Preservation International
• Subjects: Acids; Animals; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Artemia - drug effects; brine shrimp; Carbohydrate Conformation; Carbohydrate Sequence; Carbon; Cell Line, Tumor - drug effects; Cytotoxicity; Drug Screening Assays, Antitumor; Duranta repens; durantanin IV and V; Flavonoids; HepG2; Humans; Molecular Sequence Data; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Plant Leaves - chemistry; Saponins - chemistry; Saponins - isolation & purification; Saponins - pharmacology; triterpene saponins; Triterpenes - chemistry; Triterpenes - isolation & purification; Triterpenes - pharmacology; Verbenaceae - anatomy & histology; Verbenaceae - chemistry
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules14051952

From the leaves of Duranta repens (Verbenaceae) two new triterpene saponins, named durantanin IV (1) and V (2)were isolated.In addition, ten known compounds were isolated, namely a bidesmosidic saponin, oleanolic acid, three phenylethanoids and five flavonoids. All metabolites were isolated for the first time from this genus except for 3 (oleanolic acid) and 7 (E/Z acteoside). The structures were determined mainly by spectroscopic methods (UV, IR, HRESI-MS, (1)H-, (13)C-NMR, (1)H-(1)H COSY, HSQC and HMBC). Cytotoxic screening of the chloroform, ethyl acetate and methanol extracts was carried out on brine shrimps. In addition, the investigated methanol extract and compounds 1, 2 and 7 showed significant cytotoxic activity against a HepG2 cell line.