Divalent counterion-induced condensation of triple-strand DNA

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 107; no. 50; pp. 21482 - 21486
• Main Authors: Qiu, Xiangyun, Parsegian, V. Adrian, Rau, Donald C., Baldwin, Robert
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 14.12.2010; National Acad Sciences
• Subjects: Animals; Biological Sciences; Cations; Cations, Divalent - chemistry; Cells; Chickens; Condensation; Deoxyribonucleic acid; Divalent cations; DNA; DNA - chemistry; Electrostatics; Ions; Nucleic Acid Conformation; Nucleic acids; Polyelectrolytes; rev genes; Ribonucleic acid; RNA; Salts; Temperature; Thermodynamics; X ray diffraction
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.1003374107

Understanding and manipulation of the forces assembling DNA/RNA helices have broad implications for biology, medicine, and physics. One subject of significance is the attractive force between dsDNA mediated by polycations of valence ≥3. Despite extensive studies, the physical origin of the "like-charge attraction" remains unsettled among competing theories. Here we show that triple-strand DNA (tsDNA), a more highly charged helix than dsDNA, is precipitated by alkaline-earth divalent cations that are unable to condense dsDNA. We further show that our observation is general by examining several cations (Mg²⁺, Ba²⁺, and Ca²⁺) and two distinct tsDNA constructs. Cation-condensed tsDNA forms ordered hexagonal arrays that redissolve upon adding monovalent salts. Forces between tsDNA helices, measured by osmotic stress, follow the form of hydration forces observed with condensed dsDNA. Probing a well-defined system of point-like cations and tsDNAs with more evenly spaced helical charges, the counterintutive observation that the more highly charged tsDNA (vs. dsDNA) is condensed by cations of lower valence provides new insights into theories of polyelectrolytes and the biological and pathological roles of tsDNA. Cations and tsDNAs also hold promise as a model system for future studies of DNA—DNA interactions and electrostatic interactions in general.