Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid)

• Published in: Biotechnology letters Vol. 27; no. 1; pp. 53 - 58
• Main Authors: HAN, Dong-Wook, MIN SUB LEE, BONG JOO PARK, JEONG KOO KIM, PARK, Jong-Chul
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 2005; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Biotechnology; Cells, Cultured; Collagens; Differentiation; Fibronectin; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Glycolates - chemistry; Immunohistochemistry; Lactic Acid; Laminin; Localization; Microscopy, Electron, Scanning; Neurites; Neurons - cytology; Neurons - ultrastructure; Polyglycolic Acid; Position (location); Rats; Surface Properties; Neurite; Rat; Rodentia; Polymer; Cell surface; rat neural cortical cell; Vertebrata; Mammalia; Glycolic acid; Extracellular matrix; Lactic acid; neurite outgrowth; poly(lactic-co-glycolic acid)
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-004-6585-2

Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for both neurofilament and MAP-2 with well-organized neurites and somatodendritic localization. The presence of PDL with LN or FN on the PLGA films was essential for increased neural cell growth. Also, PLGA films coated with either PDL/LN or PDL/FN mixtures had higher neurite outgrowth and regular differentiation.