Research on pharyngeal bacterial flora in transoral atlantoaxial operation and the postoperative follow-up study

• Published in: BMC musculoskeletal disorders Vol. 23; no. 1; pp. 1 - 922
• Main Authors: Zhang, Yu, Fu, Suo-Chao, Wu, You, Zhou, Chu-Song, Ma, Xiang-Yang
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 19.10.2022; BioMed Central; BMC
• Subjects: Anesthesia; Atlanto-axial joint; Bacteria; Bone grafts; Bone surgery; Bone tumors; Care and treatment; Central nervous system diseases; Computed tomography; Craniovertebral junction; Disinfection; Ethics; Fractures; Health aspects; Hospitals; Infection; Infection prevention; Infections; Inflammation; Intubation; Microflora; Mouth; Mucosa; Musculoskeletal diseases; Orthopedics; Patient outcomes; Patients; Pharynx; Physiological aspects; Prevention; Prospective study; Rheumatoid arthritis; Risk factors; Spinal cord injuries; Spinal lesion; Spine; Spine (cervical); Surgery; Transoral approach; Trauma; Vertebrae; China
• ISSN: 1471-2474; 1471-2474
• DOI: 10.1186/s12891-022-05851-3

Abstract Objectives To study the changes of bacterial flora after a series of preoperative oral disinfection and the postoperative recovery of nerve function of patients with craniovertebral junction disorders who were treated with transoral approach operations. Methods This research analyzed 20 cases collected from October 2009 to May 2010. All these patients were with CVJ disorders, including 8 males and 12 females, aged 2 to 66 (38.1 on average), and they were all treated with transoral approach operations. The mucosa samples of the posterior pharyngeal wall were sent for bacteria culture. These samples were collected by sterile cotton swabs at four crucial points, including 3 days before operation/before gargling, 3 days after continuous gargling/after anesthesia intubation on the day of operation, after intraoperative cleaning and washing of the mouth, and after intraoperative iodophor immersion. The microflora was stained by means of smear and further counted after an investigation by microscope. The neural function of patients was evaluated by the ASIA classification and the JOA scores. All patients but two with posterior stabilization performed respectively underwent transoral atlantoaxial reduction plate (TARP) fixation consecutively in the same sitting. A regular reexamination of cervical vertebra with lateral and open mouth X-ray, CT and MRI was conducted after operation to evaluate the reduction of atlantoaxial dislocation, internal fixation position, bone graft fusion, inflammatory lesions and tumor recurrence. Results This bacteriological research showed that the mucosa of the posterior pharyngeal wall of all the patients was in a sterile state after a series of oral preoperative preparations and intraoperative iodophor disinfection, which was considered as type I incision. The bacterial culture results of the mucosa samples of the posterior pharyngeal wall collected at different time points showed significant differences (χ 2  = 42.762, P  = 0.000). All the patients had improvement in ASIA, and their neural functions were improved to different levels after operation. There was a significant difference in JOA scores before and after operation ( t  = 8.677, P  = 0.000). Postoperative imaging examination showed that the atlantoaxial screw position was good and firm, and the CVJ disorders were treated appropriately. Conclusion It is safe and effective to cut the posterior pharyngeal muscle layer and implant internal fixation by means of transoral approach.