A novel enzymatic approach to nanocrystalline cellulose preparation
•NCC were produced with a greener technology.•Sulfuric acid can be reduced with a cellulase pretreatment.•The optimal conditions for enzymatic treatment were established.•The enzymatic treatment time was reduced.•Enzymatic effects on fibers led to proportional differences in NCC. In this work, condi...
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Published in | Carbohydrate polymers Vol. 189; pp. 39 - 47 |
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Main Authors | , , , |
Format | Journal Article Publication |
Language | English |
Published |
England
Elsevier Ltd
01.06.2018
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Subjects | |
Online Access | Get full text |
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Summary: | •NCC were produced with a greener technology.•Sulfuric acid can be reduced with a cellulase pretreatment.•The optimal conditions for enzymatic treatment were established.•The enzymatic treatment time was reduced.•Enzymatic effects on fibers led to proportional differences in NCC.
In this work, conditions for an enzymatic pretreatment prior to NCC isolation from cotton linter were assessed. Different cellulase doses and reaction times were studied within an experimental design and NCC were obtained. At optimal enzymatic conditions (20U, 2 h), a total yield greater than 80% was achieved and the necessary enzymatic treatment time was reduced 90%. Different intensities of enzymatic treatments led to proportional decreases in fiber length and viscosity and also were inversely proportional to the amount of released oligosaccharides. These differences within fibers lead to quantitative differences in NCC: increase in acid hydrolysis yield, reduction of NCC surface charge and crystallinity increase. Benefits produced by enzymatic treatments did not have influence over other NCC characteristics such as their sulfur content (≈1%), size (≈200 nm), zeta potential (≈−50 mV) or degree of polymerization (≈200). Evidence presented in this work would reduce the use of harsh sulfuric acid generating a cleaner stream of profitable oligosaccharides. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2018.02.015 |