Characterization of extended-spectrum beta-lactamases, antimicrobial resistance genes, and plasmid content in Escherichia coli isolates from different sources in Rio de Janeiro, Brazil

• Published in: Diagnostic microbiology and infectious disease Vol. 74; no. 1; pp. 91 - 94
• Main Authors: Queiroz, Mara L.P, Antunes, Patrícia, Mourão, Joana, Merquior, Vânia L.C, Machado, Elisabete, Peixe, Luísa Vieira
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.09.2012; Elsevier
• Subjects: Anti-Bacterial Agents - pharmacology; Bacteriology; beta-Lactamases - genetics; beta-Lactamases - secretion; Biological and medical sciences; Brazil; Cluster Analysis; Community-Acquired Infections - microbiology; Cross Infection - microbiology; CTX-M-2; CTX-M-59; CTX-M-9; ESBLs; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genotype; Humans; IncA/C; Infectious Disease; Infectious diseases; Internal Medicine; Medical sciences; Microbial Sensitivity Tests; Microbiology; Miscellaneous; Molecular Typing; Phenotype; Phylogeny; Plasmids - analysis; South America; Brazil; America; ESBLs; CTX-M-59; CTX-M-2; IncA/C; CTX-M-9; Escherichia coli; Extended-spectrum β-lactamase; Antimicrobial agent; Infection; Plasmid; Resistance; Gene; Bacteria; Isolate; Enterobacteriaceae
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2012.05.019

Abstract We analyse the diversity of extended-spectrum beta-lactamases (ESBLs), antibiotic resistance genes, and plasmid content among Escherichia coli from a community and a hospital setting in Rio de Janeiro, Brazil. ESBL producers (CTX-M-2, CTX-M-9, CTX-M-59) belonged to different phylogroups/sequence types, and blaCTX-M were identified in IncA/C plasmids, reinforcing the possible intraplasmid evolution of blaCTX-M-59 from blaCTX-M-2 and the implication of IncA/C on blaCTX-M spread.