Expression of non-neuronal cholinergic system in osteoblast-like cells and its involvement in osteogenesis

• Published in: Cell and tissue research Vol. 338; no. 2; pp. 203 - 215
• Main Authors: En-Nosse, Maryam, Hartmann, Sonja, Trinkaus, Katja, Alt, Volker, Stigler, Brigitte, Heiss, Christian, Kilian, Olaf, Schnettler, Reinhard, Lips, Katrin Susanne
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.11.2009; Springer-Verlag; Springer Nature B.V
• Subjects: Acetylcholine; Animals; Biomedical and Life Sciences; Biomedicine; Bone Morphogenetic Protein 2 - metabolism; Bone Remodeling; Bones; Butyrylcholinesterase - genetics; Butyrylcholinesterase - metabolism; Carnitine Acyltransferases - genetics; Carnitine Acyltransferases - metabolism; Cell Line; Cellular biology; Enzymes; Fracture Healing; Human Genetics; Humans; Mice; Molecular biology; Molecular Medicine; Organic Cation Transport Proteins - genetics; Organic Cation Transport Proteins - metabolism; Osteoblasts - cytology; Osteoblasts - metabolism; Osteogenesis; Proteomics; Rats; Receptors, Muscarinic - genetics; Receptors, Muscarinic - metabolism; Receptors, Nicotinic - genetics; Receptors, Nicotinic - metabolism; Regular Article; Rodents; Up-Regulation; Osteoblast; Rat (Wistar, adult male); Bone morphogenetic protein-2; Fracture; Nicotinic receptor; Non-neuronal cholinergic system
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-009-0871-1

Acetylcholine (ACh) is detected in a variety of non-neuronal cells where it acts as a para/autocrine signaling molecule controlling basic cell functions such as proliferation, differentation, and maintenance of cell-cell contacts. ACh-synthesizing enzymes include choline acetyltransferase and carnitine acetyltransferase (CarAT). ACh is released through vesicular exocytosis or directly from the cytoplasm via organic cation transporters (OCT). Extracellular ACh binds to nicotinic (nAChR) and muscarinic receptors (MR). Degradation of ACh is performed by acetylcholinesterase and butyrylcholinesterase (BChE). Here, we have determined whether these molecules are expressed in osteoblast-like cells, by means of reverse transcription polymerase chain reaction and immunohistochemistry, focusing on nAChR subunits α3 and α5. RNA for CarAT, OCT-1, M2R, M5R, nAChR subunits α3, α5, α9, α10, β2, β3, and BChE were detected in human (SAOS-2) and murine (MC3T3-E1) osteoblast-like cells. Other cholinergic components were only expressed species-specifically, e.g., M3R and nAChR subunit α7. Immunhistochemistry localized the nAChR subunits α3 and α5 in osteoblasts in vitro and in vivo where they were up-regulated after application of bone morphogenetic protein-2 (BMP-2) during fracture healing in a rat model. Thus, the cholinergic system of osteoblast-like cells might be regulated by BMP-2 during bone remodeling. Osteoblast-like cells express all necessary enzymes, transporters, and receptors for ACh synthesis and recycling.