Microflora Profiling of Infected Root Canal before and after Treatment Using Culture-Independent Methods

• Published in: The journal of microbiology Vol. 50; no. 1; pp. 58 - 62
• Main Authors: Ito, Yasuhiro, Tohoku University Graduate School of Dentistry, Sendai, Japan, Sato, Takuichi, Tohoku University Graduate School of Dentistry, Sendai, Japan, Yamaki, Keiko, Tohoku University Graduate School of Dentistry, Sendai, Japan, Mayanagi, Gen, Tohoku University Graduate School of Dentistry, Sendai, Japan, Hashimoto, Kazuhiro, Tohoku University Graduate School of Dentistry, Sendai, Japan, Shimauchi, Hidetoshi, Tohoku University Graduate School of Dentistry, Sendai, Japan, Takahashi, Nobuhiro, Tohoku University Graduate School of Dentistry, Sendai, Japan
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.02.2012; Springer Nature B.V; 한국미생물학회
• Subjects: 16S ribosomal RNA; Adult; Aged; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biodiversity; Biomedical and Life Sciences; Cloning; Dental Pulp Cavity - microbiology; Dentin; Dentistry; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Endodontics; Female; Females; Fistula; FLORA MICROBIANA; FLORE MICROBIENNE; Genes; Gum disease; Humans; Laboratories; Life Sciences; Male; MICROBIAL FLORA; Microbiology; Middle Aged; Molecular Sequence Data; PCR; RNA, Ribosomal, 16S - genetics; root canals; Sequence Analysis, DNA; Teeth; 생물학; United States > US; Japan; root canals; 16S ribosomal RNA; PCR; microflora
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-012-0459-4

This study aimed to profile the microflora in infected root canals before and after root canal treatment using cultureindependent methods. Six infected root canals in singlerooted teeth with periapical lesions from five subjects were included. Quantification of total bacteria was performed by real-time PCR with primers targeting 16S rRNA genes. PCR products with universal 16S rRNA gene primers were cloned and partially sequenced, and bacterial identification at the species level was performed by comparative analysis with the GenBank database. The concentration of extracted DNA before treatment was higher than that after root canal treatment, although the difference was not statistically significant. Sequence analysis revealed that oral bacteria such as Fusobacterium, Streptococcus, Olsenella, and Pseudoramibacter detected in cases before root canal treatment disappeared after treatment. These results suggest that the root canal microflora are distinct before and after root canal treatment, and that treatment changes the microflora in both quantity and quality.