Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells

• Published in: Genomics, proteomics & bioinformatics Vol. 11; no. 5; pp. 304 - 311
• Main Authors: Wang, Juan, Gu, Qi, Hao, Jie, Bai, Donghui, Liu, Lei, Zhao, Xiaoyang, Liu, Zhonghua, Wang, Liu, Zhou, Qi
• Format: Journal Article
• Language: English
• Published: China Elsevier Ltd 01.10.2013; College of Life Science, Northeast Agricultural University of China, Harbin 150030, China%State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China%State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Graduate School of Chinese Academy of Sciences, Beijing 100049, China%College of Life Science, Northeast Agricultural University of China, Harbin 150030, China; Elsevier
• Subjects: Biomarkers - metabolism; blood; Cell Culture Techniques - methods; Cell Differentiation; Cell Separation - methods; Dox-inducible lentiviral system; embryonic stem cells; Fetal Blood - cytology; Fetal Blood - metabolism; Germ Layers - cytology; Germ Layers - metabolism; Human iPSCs; Human UCBMCs; Humans; induced pluripotent stem cells; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Infant, Newborn; iPS; Lentivirus - genetics; medicine; neonates; Original Research; patients; therapeutics; umbilical cord; 人类胚胎干细胞; 免疫学; 单个核细胞; 多能干细胞; 新生儿; 脐血; 诱导; Dox-inducible lentiviral system; Human iPSCs; Human UCBMCs
• ISSN: 1672-0229; 2210-3244
• DOI: 10.1016/j.gpb.2013.08.002

Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.